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首页> 外文会议>Conference on horticulture science and technology >Genetic Diversity and Population Structure in Amygdalus Ledebouriana Schlecht.Using Sequence-related Amplified Polymorphism Markers
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Genetic Diversity and Population Structure in Amygdalus Ledebouriana Schlecht.Using Sequence-related Amplified Polymorphism Markers

机译：利用序列相关的扩增多态性标记研究扁桃体的遗传多样性和种群结构

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The genetic diversity of 120 Amygdalus ledebouriana Schlecht. individuals from 5 populations in Xinjiang was investigated by using sequence-related amplified polymorphism (SRAP) markers. 137 bands were amplified by II informative and reliable primer combinations, of which 126 were polymorphic loci. At species level, percentage of polymorphic loci (PPL) were 91.97%, Nei's gene diversity (h) equaled to 0.3424, Shannon's Information index (1) was 0.5056; At population level, the average value of PPL was 62.92%; the mean value ofh amounted to 0.2612. and the general average of I was 0.3783. Genetic coefficient of differentiation (Gst) among populations based Net's gene diversity equaled to 0.2370. which showed 23.7% genetic variation existing among 5 populations. The mean value of Nei's genetic identity equaled to 0.8802, estimate of gene flow from Gst (Nm) was 1.6094. which was able to be considered that gene flow was unblocked among 5 populations, and this result was uniform with that obtained by using Nei's gene diversity. UPGMA dendrogram based Nei's genetic distance showed that genetic distance among populations was not to have significant correlation with geographic distance. The conclusion shows that germ plasm protection and exploitation strategies of Amygdalus ledebouriana Schlecht. should be prior to preserve available population, especially, to strengthen the screening and reserve the mutation of BRJpopulation.
机译：120个扁桃（Amygdalus ledebouriana Schlecht）的遗传多样性。使用序列相关的扩增多态性（SRAP）标记，对新疆5个种群的个体进行了调查。通过II引信和可靠的引物组合扩增了137条条带，其中126条为多态位点。在物种水平上，多态性位点（PPL）的百分比为91.97％，Nei的基因多样性（h）等于0.3424，Shannon的信息指数（1）为0.5056;在人口水平上，PPL的平均值为62.92％。 h的平均值为0.2612。而我的总平均值为0.3783。基于Net的基因多样性的人群之间的遗传分化系数（Gst）等于0.2370。这表明5个种群之间存在23.7％的遗传变异。 Nei遗传同一性的平均值等于0.8802，来自Gst（Nm）的基因流量估计为1.6094。可以认为在5个种群中基因流是畅通的，这一结果与使用Nei的基因多样性获得的结果是一致的。基于UPGMA树状图的Nei遗传距离表明，种群之间的遗传距离与地理距离没有显着相关性。结论表明，扁桃的种质保护和开发策略。应该在保留可用种群之前，特别是加强筛选和保留BRJ人群的突变之前。

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