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首页> 外文会议>China-Japan-Korea joint symposium on enzyme engineering >Enhanced Production of Recombinant Thermobifida fusca Cutinase by TIR Degeneracy Mutagenesis
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Enhanced Production of Recombinant Thermobifida fusca Cutinase by TIR Degeneracy Mutagenesis

机译：通过TIR简并诱变提高重组嗜热生双歧菌角质酶的产量

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@@Introduction As the threshold of an intracellular translation process, the Translational Initial Region (TIR) plays a constant key role in determining the whole efficiency of protein translation. Tiny alternations in TIR which extends from immediately from upstream of the Shine-Dalgarno sequence to approximately twenty nucleotides downstream of the initiation codon were reported to change the subsequent downstream translation process (1). Such property provides not only the importance of TIR to the global translation efficiency, but also a powerful potential tool for optimization of a specific recombinant protein. In present study, degeneracy mutagenesis was applied in pelB which is the most widely used signal peptide in E.coli for extracellular production of recombinant protein to enhance the expression level of cutinase from Thermobifida fusca.
机译：引言作为细胞内翻译过程的门槛，翻译起始区（TIR）在确定蛋白质翻译的整体效率中起着恒定的关键作用。据报道，TIR的微小变化从Shine-Dalgarno序列的上游直接延伸到起始密码子的下游约20个核苷酸，从而改变了随后的下游翻译过程（1）。这种特性不仅提供了TIR对全球翻译效率的重要性，而且还提供了优化特定重组蛋白的强大潜在工具。在本研究中，简并诱变应用于pelB中，而pelB是大肠杆菌中使用最广泛的信号肽，用于细胞外产生重组蛋白，以增强深部嗜热菌中角质酶的表达水平。

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