首页>
外国专利>
Method for Enhancing Extracellular Secretion of Recombinant Proteins in Escherichia coli by Co-expressing Thermobifida Fusca Cutinase
Method for Enhancing Extracellular Secretion of Recombinant Proteins in Escherichia coli by Co-expressing Thermobifida Fusca Cutinase
展开▼
机译:通过共表达嗜热菌福斯卡角质酶增强大肠杆菌中重组蛋白的细胞外分泌的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli. Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.
展开▼