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首页> 外文会议>International symposium on emerging technologies of pulping and papermaking;ISETPP >LACCASE PRODUCTION BY PSATHYRELLA CANDOLLEANA IN A STIRRED-TANK FERMENTOR
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LACCASE PRODUCTION BY PSATHYRELLA CANDOLLEANA IN A STIRRED-TANK FERMENTOR

机译：罐装发酵罐中番石榴叶霉的产胶酶。

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The laccase production by a white rot fungus, Psathyrella candolleana, was studied in a 5L stirred-tank fermentor. In order to enhance laccase production, Cu2+ and several inducers were applied during fermentation, among which Cu2+ led, by far, to the highest activity (about 23000U/L) in flask culture. The fermentation for laccase production was carried out in 5L fermentor with 1mM CuSO4·5H2O, in which the maximal laccase activity reached to about 9100U/L. The optimum activity of this laccase with ABTS as a substrate was obtained at 45°C and pH 4.0. It could retain more than 95% of its activity after 40d storage at 4°C and pH 8.0, which showed that the laccase from P. candolleana had relatively high stability.
机译：在5升搅拌罐发酵罐中研究了白腐真菌Psathyrella candolleana产生的漆酶。为了提高漆酶的产量，在发酵过程中使用了Cu2 +和几种诱导剂，其中到目前为止，Cu2 +导致烧瓶培养中的最高活性（约23000U / L）。产漆酶的发酵是在5L发酵罐中用1mM CuSO4·5H2O进行的，最大漆酶活性达到约9100U / L。以ABTS为底物的这种漆酶的最佳活性是在45°C和pH 4.0下获得的。在4°C和pH 8.0下储存40天后，它可以保留超过95％的活性，这表明来自加拿大假单胞菌的漆酶具有相对较高的稳定性。

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