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首页> 外文会议>International conference on environmental biotechnology and materials engineering;EBME 2011 >Study on the biological activity of anti-tumor peptide of Tumstatin
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Study on the biological activity of anti-tumor peptide of Tumstatin

机译：塔姆他汀抗肿瘤肽的生物活性研究

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Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma cell. To study its effect on the proliferation and apoptosis of different tumor cells and verify its anti-tumor non-specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT assay, cell growth curve, TUNEL assay. flow cytometry, transmission electron microscopy(TEM), the biological activity of 19peptide was studied. Experiments in vitro identified that obtained 19peptide could inhibit proliferation of hela cell and hepatocarcinoma cell. It also could promote two tumor cells apoptosis. Its anti-tumor effect will lay foundation on its mechanism of action research and clinically tumor therapy in future.
机译：Tumstatin抗肿瘤肽19肽可抑制黑色素瘤细胞的增殖。为了研究其对不同肿瘤细胞的增殖和凋亡的影响并验证其抗肿瘤非特异性，设计并构建了工程化细菌19肽的碱基序列。可溶性19肽是从几步几丁质亲和色谱中获得的。通过MTT法，细胞生长曲线，TUNEL法等实验。流式细胞仪，透射电镜（TEM），19肽的生物学活性进行了研究。体外实验发现，所获得的19肽可抑制hela细胞和肝癌细胞的增殖。它还可以促进两个肿瘤细胞的凋亡。其抗肿瘤作用将在未来的作用机理研究和临床肿瘤治疗方面奠定基础。

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