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Tumstatin 7 Peptide affect Biological Activity of B16 melanoma Cell

机译:Tumstatin 7肽影响B16黑色素瘤细胞的生物学活性

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To study the effect of biological activity of Tumstatin 7 peptide on the cell proliferation and apoptosis of B 16 melanoma.Tumstatin 7 peptide was synthesized and its purity is 98.4532% by high-performance liquid chromatography.The effect of 7 peptide on B16 melanoma was observed by MTT,cell growth curve,the observation of the transmission electron microscope (TEM).7 peptide can significantly inhibit B16 melanoma cell proliferation,showing dose-and time-dependent.Its IC50 was 72.53 μg/ml.The morphology ofB 16 melanoma cell was obviously changed by TEM,such as karyopyknosis and apoptotic bodies.So7 peptides can inhibit the proliferation of melanoma cells and promote melanoma cells apoptosis.It will be of great potential value to melanoma treatment.
机译:为了研究Tumstatin 7肽的生物活性对B 16黑色素瘤细胞增殖和凋亡的影响。通过高效液相色谱法合成了Tumstatin 7肽,其纯度为98.4532%。观察了7种肽对B16黑色素瘤的作用。 MTT法,细胞生长曲线,透射电镜观察。7肽可明显抑制B16黑色素瘤细胞增殖,呈剂量和时间依赖性,IC50为72.53μg/ml。B16黑色素瘤细胞的形态透射电镜可明显地改变黑素瘤细胞的核分裂症和凋亡小体等功能。因此,So7肽可以抑制黑素瘤细胞的增殖,促进黑素瘤细胞的凋亡,对黑素瘤的治疗具有重要的潜在价值。

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