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DNA detection and characterization of nano magnetite powders synthesized by sonomechanical method

机译:南磁铁矿粉末合成的DNA检测与表征南磁铁矿粉末

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In order to detect DNA, we employed nano magnetite powders of Fe3O4. In XRD results, the nanoparticle was synthesized mainly in magnetite phase and also maghemite (r-Fe2O3) phase was trivial portion. The surface morphology of the powders observed that the particle size was about 40 nm and did not change as varying annealing temperature. In agarose gel electrophoresis analysis, after bonding DNA, Fe2O3 and Fe3O4 nanopaticles showed the fixed state in the range of lane 2∼4. But the mixed particles were very weak reaction when compared with Fe3O4. Additionally, Fe3O4 presented the reaction of DNA in lane 5∼7. From these results, we confirmed a chance of DNA detection by using the nanoparticle.
机译:为了检测DNA,我们采用Fe 3 O 4 的纳米磁铁矿粉末。在XRD结果中,主要在磁铁矿相中合成纳米颗粒,并且还合成磁石(R-FE 2 O 3 )相是微不足平的部分。粉末的表面形态观察到粒径为约40nm,并且不随变化的退火温度而变化。在琼脂糖凝胶电泳分析中,粘合DNA,Fe 2 O 3 和FE 3 O 4 纳米型显示固定状态在车道2~4的范围内。但与Fe 3 O 4 相比,混合颗粒非常弱。此外,Fe 3 O 4 呈现DNA在泳道5~7中的反应。从这些结果中,我们通过使用纳米颗粒确认了DNA检测的机会。

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