In this paper, we developed injection of a single fluorescent nanosensor into a specific cell by using membrane fusion of liposome and optical tweezers for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using spontaneous transfer of phospholipid-coated sensor through an oil/water interface. The sensor is manipulated by optical tweezers and injected into the cell by membrane fusion of the liposome. We also evaluated measurement accuracy of temperature from temperature calibration by fluorescent intensity and the error of measurement point. We demonstrated the fabrication of the liposome containing single sensor, immobilization of the sensor to cellular membrane, and injection of the sensor into the cell cytoplasm and nucleus.
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