首页> 外国专利> rna targeting dna; DNA polynucleotide; recombinant expression vector; genetically modified host cell in vitro; variant site-directed modification polypeptide; chimeric site-directed modification polypeptide; rna polynucleotide; genetically modified cell; transgenic non-human organism whose genome comprises a transgene; composition; site specific modification method of a target DNA; site specific transcriptional modulation method within a target DNA; site-specific modification method in target DNA; method of promoting site-specific cleavage and modification of a target DNA in a cell; method of producing a genetically modified cell in a subject; method of modifying target DNA in a genetically modified cell; kit; target dna target kit; method of selectively modulating transcription of a target DNA into a host cell; isolated nucleic acid; genetically modified host cell; and nucleic acid library

rna targeting dna; DNA polynucleotide; recombinant expression vector; genetically modified host cell in vitro; variant site-directed modification polypeptide; chimeric site-directed modification polypeptide; rna polynucleotide; genetically modified cell; transgenic non-human organism whose genome comprises a transgene; composition; site specific modification method of a target DNA; site specific transcriptional modulation method within a target DNA; site-specific modification method in target DNA; method of promoting site-specific cleavage and modification of a target DNA in a cell; method of producing a genetically modified cell in a subject; method of modifying target DNA in a genetically modified cell; kit; target dna target kit; method of selectively modulating transcription of a target DNA into a host cell; isolated nucleic acid; genetically modified host cell; and nucleic acid library

机译：针对dna的rna; DNA多核苷酸;重组表达载体体外转基因宿主细胞;变体定点修饰多肽;嵌合定点修饰多肽; rna多核苷酸;转基因细胞基因组包含转基因的转基因非人类生物;组成;靶DNA的位点特异性修饰方法;靶DNA内的位点特异性转录调节方法;靶DNA的位点特异性修饰方法;促进细胞中靶DNA的位点特异性切割和修饰的方法;在受试者中产生基因修饰的细胞的方法;在转基因细胞中修饰靶DNA的方法;套件目标dna目标试剂盒;选择性调节靶DNA转录到宿主细胞中的方法;分离的核酸转基因宿主细胞;和核酸文库

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rna summary targeting dna; DNA polynucleotide; recombinant expression vector; genetically modified host cell in vitro; variant site-directed modification polypeptide; chimeric site-directed modification polypeptide; rna polynucleotide; genetically modified cell; transgenic non-human organism whose genome comprises a transgene; composition; site specific modification method of a target DNA; site specific transcriptional modulation method within a target DNA; site-specific modification method in target DNA; method of promoting site-specific cleavage and modification of a target DNA in a cell; method of producing a genetically modified cell in a subject; method of modifying target DNA in a genetically modified cell; kit; target dna target kit; method of selectively modulating transcription of a target DNA into a host cell; isolated nucleic acid; genetically modified host cell; and nucleic acid library the present disclosure provides a DNA targeting DNA comprising a target sequence and, together with the modification polypeptide, provides site specific modification of a target DNA and / or an associated polypeptide such as a DNA. target. The present disclosure further provides site specific modification polypeptides. The present disclosure further provides site specific modification methods of a target DNA and / or a polypeptide associated with the target DNA. The present disclosure provides methods of modulating transcription of a target nucleic acid into a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive cas9 polypeptide and an RNA targeting DNA. Kits and compositions for performing the methods are also provided. the present disclosure provides genetically engineered cas9 producing cells; and transgenic non-human multicellular organisms cas9.

机译：针对dna的rna摘要; DNA多核苷酸;重组表达载体体外转基因宿主细胞;变体定点修饰多肽;嵌合定点修饰多肽; rna多核苷酸;转基因细胞基因组包含转基因的转基因非人类生物;组成;靶DNA的位点特异性修饰方法;靶DNA内的位点特异性转录调节方法;靶DNA的位点特异性修饰方法;促进细胞中靶DNA的位点特异性切割和修饰的方法;在受试者中产生基因修饰的细胞的方法;在转基因细胞中修饰靶DNA的方法;套件目标dna目标试剂盒;选择性调节靶DNA转录到宿主细胞中的方法;分离的核酸转基因宿主细胞;本发明提供了一种DNA靶向DNA，其包含靶序列，并与修饰多肽一起提供靶DNA和/或相关多肽例如DNA的位点特异性修饰。目标。本公开进一步提供位点特异性修饰多肽。本公开进一步提供靶DNA和/或与靶DNA相关的多肽的位点特异性修饰方法。本公开内容提供了调节靶核酸转录到靶细胞中的方法，该方法通常包括使靶核酸与酶促无活性的cas9多肽和靶向RNA的DNA接触。还提供了用于执行该方法的试剂盒和组合物。本公开提供了基因工程的cas9产生细胞;和转基因非人类多细胞生物体cas9。

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公开/公告号BR112014029441A2

专利类型
公开/公告日2017-06-27

原文格式PDF
申请/专利权人 EMMANUELLE CHARPENTIER;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;UNIVERSITY OF VIENNA;
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申请/专利号BR20141129441
发明设计人 EMMANUELLE CHARPENTIER;JAMES HARRISON DOUDNA CATE;JENNIFER A. DOUDNA;KRZYSZTOF CHYLINSKI;LEI QI;MARTIN JINEK;WENDELL LIM;
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申请日2013-03-15
分类号C07K19;C12N5/10;C12N15/11;C12N15/63;
国家 BR
入库时间 2022-08-21 13:40:24

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