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Acetylcholine dynamics in cortical networks by an ion image sensor with neurotransmitter-sensitive magnetic nanomachines

机译:带有神经递质敏感型磁性纳米机的离子图像传感器在皮质网络中的乙酰胆碱动力学

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To investigate the secretory properties in the neuronal networks, we developed the ion image sensor-based acetylcholine (ACh) imaging system utilizing with the ACh esterase-tagged magnet bead complexes. The magnetic particles were successfully placed only at the well of each sensing area of an ion image sensor (128 × 128 pixels type, 23 μπι pixel pitch). The pH value decreased immediately after the addition of ACh in the medium, and the degree of pH decrease was dependent on the dose of ACh. The ACh-dependent pH decrease was also detectable in the neurons of the cortical slice stimulated with glutamate. The newly developed chemical microscopy demonstrated the 2D real-time analysis of the ACh release in the non-labelled neurons of the brain network.
机译:为了研究神经元网络中的分泌特性,我们开发了基于离子图像传感器的乙酰胆碱(ACh)成像系统,该系统与ACh酯酶标记的磁珠复合物一起使用。仅将磁性颗粒成功地放置在离子图像传感器的每个感应区域的阱处(128×128像素类型,23μm像素间距)。在培养基中添加ACh后,pH值立即下降,pH下降的程度取决于ACh的剂量。在谷氨酸刺激的皮质切片的神经元中,也可检测到ACh依赖性的pH降低。新开发的化学显微镜证明了大脑网络未标记神经元中ACh释放的2D实时分析。

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