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首页> 外文会议>Annual International Conference of the IEEE Engineering in Medicine and Biology Society >Whole Organ Decellularization - A Tool for Bioscaffold Fabrication and Organ Bioengineering
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Whole Organ Decellularization - A Tool for Bioscaffold Fabrication and Organ Bioengineering

机译：整个器官脱细胞化 - Bioscaffold制造和器官生物工具

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摘要

The use of synthetic and naturally-derived scaffolds for bioengineering of solid organs has been limited due to a lack of an integrated vascular network. Here, we describe fabrication of a bioscaffold system with intact vascular tree. Animal-donor organs and tissues, ranging in size up-to thirty centimeters, were perfused with decellularization solution to selectively remove the cellular component of the tissue and leave an intact extracellular matrix and vascular network. The vascular tree demonstrated sequential fluid flow through a central inlet vessel that branched into an extensive capillary bed and coalesced back into a single outlet vessel. In one example, the liver, we used central inlet vessels to perfuse human and animal liver cells through the bioscaffold to create a functional liver tissue construct in vitro. These results demonstrate a novel yet simple and scalable method to obtain whole organ vascularized bioscaffolds with potential for liver, kidney, pancreas, intestine and other organs' bioengineering. These bioscaffolds can further provide a tool to study cells in their natural three-dimensional environment, which is superior for drug discovery platform compared with cells cultured in two-dimensional petri dishes.
机译：由于缺乏综合的血管网络，使用用于生物工程的合成和天然衍生的支架的用途受到限制。在这里，我们描述了具有完整血管树的生物支出系统的制造。动物供体器官和组织，大小不等向上三十厘米，去细胞溶液灌注以选择性地去除所述组织的细胞组分和离开一个完整的细胞外基质和血管网。血管树证明了顺序流体流过中央入口容器，该容器支撑成广泛的毛细管床并将其聚集回到单个出口容器中。在一个实例中，肝脏，我们使用中央入口容器来淹没人和动物肝细胞通过Bioscaffold在体外产生功能性肝组织构建体。这些结果表明了一种新颖的且简单且可扩展的方法，以获得具有肝脏，肾，胰腺，肠道和其他器官的潜力的整个器官血管化生物领带。这些Bioscadomds可以进一步提供用于研究其天然三维环境中的细胞的工具，该工具与在二维培养皿中培养的细胞相比，对药物发现平台优越。

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