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首页> 外文会议>Annual International Conference of the IEEE Engineering in Medicine and Biology Society >A New Automatic Cell Isolation System for Flow Cytometry: Cell Isolation Unit and Staining Reagent Kit
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A New Automatic Cell Isolation System for Flow Cytometry: Cell Isolation Unit and Staining Reagent Kit

机译：流式细胞术新型自动细胞隔离系统：细胞分离单元和染色试剂盒

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Flow cytometry is well-known cell analysis method and useful to gain quantitative information from cells in blood, however, it is not widely used for solid tissues in clinical settings. This is partly because it takes a long time to prepare samples and the operation can be complicated. To resolve these problems, we developed a new automatic cell isolation system which consists of cell isolation unit and staining reagent kit specialized for flow cytometry. With this new system, cell isolation can be done more rapidly and easily. By using this method, we could determine optimum condition to disintegrate porcine colon tissue and stain cells stably in 6 minutes. This result indicates that our method can provide analysis data within 10 minutes. We also evaluated our method in colorectal cancer patients, and the result was promising. All the data suggests that this method can support and facilitate rapid diagnosis.
机译：流式细胞术是公知的细胞分析方法，可用于从血液中的细胞中获得定量信息，然而，它不广泛用于临床环境中的固体组织。这部分是因为需要很长时间才能准备样本，并且操作可以复杂。为了解决这些问题，我们开发了一种新的自动细胞隔离系统，由细胞分离单元和专门用于流式细胞仪的染色试剂盒组成。通过这种新系统，可以更快，轻松地进行细胞隔离。通过使用这种方法，我们可以确定在6分钟内稳定地将猪结肠组织和染色细胞进行崩解的最佳条件。此结果表明，我们的方法可以在10分钟内提供分析数据。我们还评估了我们在结肠直肠癌患者中的方法，结果很有前景。所有数据都表明该方法可以支持和促进快速诊断。

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