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Specific CA3 neurons decode neural information of dentate granule cells evoked by paired-pulse stimulation in co-cultured networks

机译:特定Ca3神经元解码通过共培养网络中的配对脉冲刺激引起的牙齿颗粒细胞的神经信息

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CA3 and dentate gyrus (DG) neurons are cultured in two-chamber devices on multi-electrode arrays (MEAs) and connected via micro-tunnels. In order to evoke time-locked activity, paired-pulse stimulation is applied to 22 different sites and repeated 25 times in each well in 5 MEA co-cultures and results compared to CA3-CA3 and DG-DG networks homologous controls. In these hippocampal sub-regions, we focus on the mechanisms underpinning a network's ability to decode the identity of site specific stimulation from analysis of evoked network responses using a support vector machine classifier. Our results indicate that a pool of CA3 neurons is able to reliably decode the identity of DG stimulation site information.
机译:Ca3和牙齿回形物(DG)神经元在多电极阵列(MEAS)上的两个室装置中培养并通过微隧道连接。为了唤起锁定的活动,将配对脉冲刺激施加到22种不同的部位,并在5个MEA共培养物中重复25次,与CA3-CA3和DG-DG网络同源控制相比。在这些海马子区域中,我们专注于支持网络对网站特定刺激的身份的能力的机制,从使用支持向量机分类器分析诱发网络响应的分析。我们的结果表明,CA3神经元的池能够可靠地解码DG刺激现场信息的身份。

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