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Expression and Sequence Analysis of ChRpS3, a Ribosomal Protein S3 cDNA, and its Potential Role in Ovary Development of Cymbidium hybridium

机译:CHRPS3,核糖体蛋白S3 cDNA的表达及序列分析及其在卵巢杂交卵巢发育中的潜在作用

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A full length cDNA sequence, Ch-RpS3, was isolated by RT-PCR and further characterized by sequencing and expression analysis. The cDNA fragment was 1,003 bp in length and contained a complete open reading frame of 786 bp, encoding a protein of 261 amino acid residues with high sequence homology to RpS3 from other plants such as monocotyledon Oryza sativa (91 %). Phylogenetic analysis at the amino acid level also confirmed that Ch-RpS3 belonged to the monocot-specific RpS3 clade. Furthermore, spatial and temporal expression analysis with RT-PCR indicated that Ch-RpS3 was highly expressed in ovaries 2 days after pollination and is predicted to encode a novel member of RPS3 participating in cell growth and proliferation. It is deduced that this 40S ribosomal S3 like protein is involved in initiating ovary development of C. hybridium, which might provide novel insights into the molecular mechanism of ovary and floral development.
机译:通过RT-PCR分离全长cDNA序列CH-RPS3,并进一步通过测序和表达分析表征。 CDNA片段的长度为1,003bp,含有786bp的完整开放阅读框,编码了261个氨基酸残基的蛋白质,其具有高序列同源性与其他植物,如单胶质叶菊属植物(91%)。氨基酸水平的系统发育分析还证实CH-RPS3属于单子偶像特异性RPS3疏水。此外,具有RT-PCR的空间和时间表达分析表明CH-RPS3在授粉后2天在卵巢中高度表达,预计将编码参与细胞生长和增殖的RPS3的新型成员。推导出该40s的核糖体S3如蛋白质参与启动C.杂交的卵巢发育,这可能会对卵巢和花卉发育的分子机制提供新的见解。

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