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Production of Diosgenin from Dioscorea zingiberensis by Mixed Culture of Three Filamentous Fungi

机译:三种丝状真菌混合培养从薯Di中提取薯gen皂苷元

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The mixed culture of Aspergillus oryzae, Phanerochaete chrysosporium, and Aspergillus niger was explored for the production of diosgenin from Dioscorea zingiberensis. Conditions for biotransformation of saponin using mixed culture of three fungi were optimized. The maximum production of diosgenin (40.06 ± 0.53 mg/g) was obtained with D. zingiberensis concentration (50.00 g/L), inoculation size (2.0 mL of spore suspension, 1:2:3 ratio of A. oryzae, P. chrysosporium, and A. niger, v/v/v), initial pH (5.0), and cultivation time (6 days). In the optimization conditions, mono and mixed cultures were tested, with mixed culture of three fungi giving better yields of diosgenin and β-glucosidase. The diosgenin yield of optimization biotransformation increased by 50.54 % compared to the traditional acid hydrolysis method (26.61 ± 0.78 mg/g). The use of mixed culture of these fungi proposes an approach for more efficient and clean diosgenin production by D. zingiberensis.
机译:探索了米曲霉,Phanerochaete chrysosporium和黑曲霉的混合培养物,以从盾叶薯os生产薯os皂苷元。优化了使用三种真菌混合培养进行皂素生物转化的条件。在姜黄D.浓度(50.00 g / L),接种量(2.0 mL孢子悬浮液,米曲霉,金黄色葡萄球菌的比例为1:2:3)下,薯di皂甙元的最大产量(40.06±0.53 mg / g)获得。和黑曲霉(v.v / v),初始pH(5.0)和培养时间(6天)。在最优化条件下,测试了单培养和混合培养,其中三种真菌的混合培养产生薯os皂苷元和β-葡萄糖苷酶的产率更高。与传统的酸水解方法(26.61±0.78 mg / g)相比,优化生物转化的薯os皂素产率提高了50.54%。这些真菌的混合培养的使用提出了一种方法,用于更有效地和清洁地生产姜黄D.薯di皂苷元。

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