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首页> 外文会议>Conference on nanoscale imaging, sensing, and actuation for biomedical applications >Thin-layer infrared spectroscopic study on thermal behavior of non-phospholipid lipids and nanovesicles
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Thin-layer infrared spectroscopic study on thermal behavior of non-phospholipid lipids and nanovesicles

机译：非磷脂脂质和纳米粒子热行为的薄层红外光谱研究

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The investigation of thermal behaviors and subsequent changes in the conformational order of lipids and liposomes is of importance in understanding various phenomena such as the formation and fusion of vesicles, trans-membrane diffusion and membrane interactions with drugs and proteins. In this work, the thermal behavior of a suite of newly developed self-forming synthetic non-phospholipid (PEGylated) lipids and its nanovesicles in buffer suspensions were investigated by variable-temperature thin-layered Fourier Transform Infrared (FTIR) transmission spectroscopy. The temperature-induced infrared spectra of such lipids composed of 1,2-dimyristoyl-rac-glycerol-3-dodecaethylene glycol (GDM-12) and 1,2-distearoyl-rac-glycerol-3-triicosaethylene glycol (GDS-23) were acquired by using FTIR spectrometer in conjunction with a custom built temperature-controlled demountable liquid cell. In contrast to conventional phospholipids, these novel lipids form liposomes spontaneously upon hydration, without the supply of external activation energy. It was found that the thermal stability of the PEGylated lipids defer greatly depending upon the acyl chain-lengths as well as number of associated head group units. Particularly, GDM-12 (saturated 14 hydrocarbon chains) shows one sharp order-disorder transition with temperature increasing from 3 to 5 °C. Similarly, GDS-23 (saturated 18 hydrocarbon chains) exhibits comparatively broad order-disorder transition profiles between temperature 17 and 22 °C. However, the phase transition temperature becomes significantly higher for lipid nanovesicles formed in aqueous suspensions. The results obtained in this study may find applications in various areas including the development of lipid based substance and drug delivery systems.
机译：对脂质和脂质体构象顺序的热行为和随后的变化进行了重要性在理解各种现象，例如囊泡的形成和融合，跨膜扩散和药物和蛋白质相互作用的各种现象具有重要性。在这项工作中，通过可变温度薄层傅里叶变换红外（FTIR）透射光谱研究了一种新开发的自成形合成非磷脂（PEG化）脂质及其在缓冲膜中的纳米粒子的热行为。由1,2-Dimyristoyl-Rac-甘油-3-十二烷基二醇（GDM-12）和1,2-羟基酰基 - 甘油-3-三仙亚甲基二醇（GDS-23）组成的这种脂质的温度诱导的红外光谱。通过使用FTIR光谱仪结合定制的内置温控可拆卸液体电池获得。与常规的磷脂相比，这些新型脂质在不含外部激活能量的情况下自发地形成脂质体。发现Pegymated脂质的热稳定性根据酰基链长以及相关头组单元的数量大大提高。特别地，GDM-12（饱和14个烃链）显示出一种尖锐的订单障碍转变，温度从3-5℃增加。类似地，GDS-23（饱和18烃链）在温度17和22℃之间表现出相对较大的阶阶紊乱转变曲线。然而，在含水悬浮液中形成的脂质纳米粒子变得显着更高。本研究中获得的结果可以在各种区域中找到应用，包括脂基物质和药物递送系统的发育。

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