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首页> 外文会议>Nanoscale imaging, sensing, and actuation for biomedical applications VI >Thin-layer infrared spectroscopic study on thermal behavior of non-phospholipid lipids and nanovesicles
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Thin-layer infrared spectroscopic study on thermal behavior of non-phospholipid lipids and nanovesicles

机译：薄层红外光谱研究非磷脂脂质和纳米囊泡的热行为

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摘要

The investigation of thermal behaviors and subsequent changes in the conformational order of lipids and liposomes is of importance in understanding various phenomena such as the formation and fusion of vesicles, trans-membrane diffusion and membrane interactions with drugs and proteins. In this work, the thermal behavior of a suite of newly developed self-forming synthetic non-phospholipid (PEGylated) lipids and its nanovesicles in buffer suspensions were investigated by variable-temperature thin-layered Fourier Transform Infrared (FTIR) transmission spectroscopy. The temperature-induced infrared spectra of such lipids composed of l,2-dimyristoyl-rac-glycerol-3-dodecaethylene glycol (GDM-12) and 1,2-distearoyl-rac-glycerol-3-triicosaethylene glycol (GDS-23) were acquired by using FTIR spectrometer in conjunction with a custom built temperature-controlled demountable liquid cell. In contrast to conventional phospholipids, these novel lipids form liposomes spontaneously upon hydration, without the supply of external activation energy. It was found that the thermal stability of the PEGylated lipids defer greatly depending upon the acyl chain-lengths as well as number of associated head group units. Particularly, GDM-12 (saturated 14 hydrocarbon chains) shows one sharp order-disorder transition with temperature increasing from 3 to 5 ℃. Similarly, GDS-23 (saturated 18 hydrocarbon chains) exhibits comparatively broad order-disorder transition profiles between temperature 17 and 22 ℃. However, the phase transition temperature becomes significantly higher for lipid nanovesicles formed in aqueous suspensions. The results obtained in this study may find applications in various areas including the development of lipid based substance and drug delivery systems.
机译：研究热行为以及脂质和脂质体的构象顺序的后续变化对于理解各种现象（例如小泡的形成和融合，跨膜扩散以及与药物和蛋白质的膜相互作用）非常重要。在这项工作中，通过可变温度薄层傅里叶变换红外（FTIR）透射光谱研究了一组新开发的自形成合成非磷脂（PEG化）脂质及其纳米囊在缓冲液悬浮液中的热行为。由1，2-二肉豆蔻酰基-rac-甘油-3-十二烷基乙二醇（GDM-12）和1,2-二硬脂酰基-rac-甘油-3-三二十二碳六烯（GDS-23）组成的脂质的温度诱导红外光谱通过使用FTIR光谱仪与定制的温度控制可拆卸液体池结合获得。与常规磷脂相反，这些新型脂质在水合时自发形成脂质体，而无需提供外部活化能。发现聚乙二醇化脂质的热稳定性极大地取决于酰基链长度以及相关的头基单元的数量。特别地，随着温度从3℃升高到5℃，GDM-12（饱和的14条烃链）显示出一个急剧的无序转变。同样，GDS-23（饱和的18个碳氢链）在17到22℃之间表现出较宽的有序-无序过渡曲线。然而，对于在水悬浮液中形成的脂质纳米囊泡，相变温度变得明显更高。在这项研究中获得的结果可能会在各个领域找到应用，包括基于脂质的物质和药物输送系统的开发。

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