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Studies on polymer capped quantum dots and peptide self-assembly using fluorescence coupled capillary electrophoresis

机译:荧光偶联毛细管电泳研究聚合物封端的量子点和肽的自组装

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A new bioprobe was designed to study the quantum dots (QDs) based bioanalysis. Then, fluorescence coupled capillary electrophoresis (CE-FL) was utilized to probe the self-assembly of a bifunctional polymer (PAA-g-MEA) capped QDs and ATTO labeled peptide (ATTO-EA5H6). Fluorescence resonance energy transfer (FRET) was detected readily by CE-FL. The FRET signal increased with ATTO-EA5H6/QD molar ratio, which plateaued at 4:1.
机译:设计了一种新的生物探针来研究基于量子点(QD)的生物分析。然后,利用荧光耦合毛细管电泳(CE-FL)来探测双功能聚合物(PAA-g-MEA)封端的量子点和ATTO标记的肽(ATTO-EA5H6)的自组装。 CE-FL可以很容易地检测到荧光共振能量转移(FRET)。 FRET信号随ATTO-EA5H6 / QD摩尔比的增加而增加,稳定在4:1。

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