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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis
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In-capillary self-assembly study of quantum dots and protein using fluorescence coupled capillary electrophoresis

机译：荧光耦合毛细管电泳在量子点和蛋白质的毛细管内自组装研究

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摘要

As a vast number of novel materials in particular inorganic nanoparticles have been invented and introduced to all aspects of life, public concerns about how they might affect our ecosystem and human life continue to arise. Such incertitude roots at a fundamental question of how inorganic nanoparticles self-assemble with biomolecules in solution. Various techniques have been developed to probe the interaction between particles and biomolecules, but very few if any can provide advantages of both rapid and convenient. Herein, we report a systematic investigation on quantum dots (QDs) and protein self-assembly inside a capillary. QDs and protein were injected to a capillary one after another. They were mixed inside the capillary when a high voltage was applied. Online separation and detection were then achieved. This new method can also be used to study the self-assembly kinetics of QDs and protein using the Hill equation, the K-D value for the self-assembly of QDs and protein was calculated to be 8.8 M. The obtained results were compared with the previous out of-capillary method and confirmed the effectiveness of the present method.
机译：由于已经发明了许多新颖的材料，尤其是无机纳米颗粒，并将其引入到生活的各个方面，公众日益关注它们如何影响我们的生态系统和人类生活。这种不确定性源于一个基本问题，即无机纳米粒子如何与溶液中的生物分子自组装。已经开发了各种技术来探测颗粒和生物分子之间的相互作用，但是很少有（如果有的话）可以提供快速和方便的优点。在这里，我们报告了对毛细管内的量子点（QDs）和蛋白质自组装的系统研究。将QD和蛋白质一个接一个地注射到毛细管中。当施加高压时，它们在毛细管内部混合。然后实现了在线分离和检测。该新方法还可用于使用Hill方程研究量子点和蛋白质的自组装动力学，计算量子点和蛋白质的自组装的KD值为8.8M。将所得结果与以前的结果进行比较非毛细管法，并证实了本方法的有效性。

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