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首页> 外文会议>World biomaterials congress >Anti-biofilm effect of denture base resin containing dimethylaminododecyl methacrylate on multi-species biofilm
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Anti-biofilm effect of denture base resin containing dimethylaminododecyl methacrylate on multi-species biofilm

机译：含甲基丙烯酸二甲氨基十二烷基酯的义齿基托树脂对多种生物膜的抗生物膜作用

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Introduction: The increased denture stomatitis due to unbalanced interactions between oral microbiota requires novel denture materials with antimicrobial ability. Objectives: The objectives of this study were to investigate the mechanical properties and antimicrobial effect of a new denture base resin containing dimethylaminododecyl methacrylate (DMADDM) on multi-species biofilms and the biocompatibility of this material for the first time. Materials and Methods: DMADDM was mixed with one third of the specimen, at a mass fraction of 0% (control group), 1.65 %, 3.3% and 6.6 %. Candida albicans, Streptococcus mutans, Actinomyces naeslundii and Streptococcus sanguinis were chosen to form multi-species biofilms on denture base resin with DMADDM. Scanning electron microscope (SEM) imaging, colony-forming unit (CFU) counts, confocal laser scanning microscopy (CLSM) imaging analysis as well as XTT assays were used to quantify the biomass and metabolic activies change of biofilms. The effects on morphological change of C. albicans were observed by CLSM and the virulence genes expressions were quantified using real-time PCR. LDH array were used to determine the damage of epithelial cells co-cultured with the biofilm while real-time PCR were performed to quantify the gene expressions of cells responding to the biofilms. Animal experiment was performed and followed by hematoxylin-eosin (HE) staining and histological evaluation to study the biocompatibility of denture base resin in vivo. Flexure strength was measured to evaluate the mechanical properties of resin containing DMADDM. Results and Discussion: The denture base resin containing DMADDM reduced both the biomass and the metabolic activity of biofilms (p ＜ 0.05). In DMADDM-containing groups, the hyphal form of C. albicans was less prevalent while the virulence genes ALS3 and HWP1 gene were also down-regulated (p ＜ 0.05). The reduced C. albicans virulence factors in these modified groups were consistent with the down-regulation of cell IL-18 gene and lower LDH activity when the biofilm was co-cultured with TR-146 cells (p ＜ 0.05). In vivo histological evaluations proved that the addition of DMADDM at less than 6.6% in denture material did not increase the inflammatory response (p ＞ 0.05). The denture base resin with DMADDM had ftexural strength similar to that of the control group. Conclusion: The new antibacterial denture base resin with DMADDM had strong inhibition against multi-species biofilms, without compromising the mechanical properties. The novel anti-biofilm denture base resin is a new promising therapeutic system for denture stomatitis applications.
机译：简介：由于口腔微生物群之间相互作用不平衡而导致的义齿口腔炎增加，因此需要具有抗菌能力的新型义齿材料。目的：本研究的目的是首次研究一种含有甲基丙烯酸二甲基氨基十二烷基酯（DMADDM）的新型义齿基托树脂在多种生物膜上的力学性能和抗菌效果，以及该材料的生物相容性。材料与方法：将DMADDM与三分之一的样品混合，质量分数分别为0％（对照组），1.65％，3.3％和6.6％。选择白色念珠菌，变形链球菌，内生放线菌和血链球菌，用DMADDM在义齿基托树脂上形成多物种生物膜。扫描电子显微镜（SEM）成像，菌落形成单位（CFU）计数，共聚焦激光扫描显微镜（CLSM）成像分析以及XTT分析用于量化生物膜的生物量和代谢活性变化。通过CLSM观察对白色念珠菌形态变化的影响，并通过实时PCR定量毒力基因表达。 LDH阵列用于确定与生物膜共培养的上皮细胞的损伤，同时进行实时PCR定量反应生物膜的细胞的基因表达。进行动物实验，然后进行苏木精-伊红（HE）染色和组织学评估，以研究义齿基托树脂在体内的生物相容性。测量挠曲强度以评估含有DMADDM的树脂的机械性能。结果与讨论：含有DMADDM的义齿基托树脂同时降低了生物膜的生物量和代谢活性（p ＜0.05）。在含DMADDM的组中，白色念珠菌的菌丝形式不那么普遍，而毒力基因ALS3和HWP1基因也被下调（p ＜0.05）。当这些生物膜与TR-146细胞共培养时，这些修饰组中降低的白色念珠菌毒力因子与细胞IL-18基因的下调和较低的LDH活性相符（p ＜0.05）。体内组织学评估证明，在义齿材料中加入少于6.6％的DMADDM不会增加炎症反应（p＞ 0.05）。具有DMADDM的义齿基托树脂的折线强度与对照组相似。结论：新型含DMADDM的抗菌义齿基托树脂对多种生物膜具有很强的抑制作用，而不会影响其机械性能。新型的抗生物膜义齿基托树脂是用于义齿口炎的一种新的有前途的治疗系统。

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