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Modulation of keratin in adhesion, proliferation, adipogenic and osteogenic differentiation of porcine adipose-derived stem cells

机译：角蛋白对猪脂肪干细胞粘附，增殖，成脂和成骨分化的调节

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Keratin, isolated from human hair, gains researcher's interests due its intrinsic ability to interact with different cells. Keratin has the potential to serve as a controllable extracellular matrix protein and can be used to demonstrate cell mechanism and cell-matrix interaction. However, the effects of keratin on stem cells have not been reported. In the present study, the effects of keratin on porcine adipose-derived stem cells (pASCs) and a series of selective cell lines: 3T3 fibroblasts, Madin-Darby canine kidney (MOCK) cells, and MG63 osteoblasts were demonstrated. Relative to un-treated culture plate, results showed that keratin coating substrates promoted cell adhesion and proliferation to above cell lines. Keratin also improved pASCs adhesion, proliferation, and enhanced cell viability. Evaluation of genetic markers showed that adipogenic and osteogenic differentiations of pASCs can be successfully induced, which revealed the keratin did not influence the sternness of pASCs. In addition, keratin improved adipogenic differentiations in terms of up-regulations in lipoprotein lipase, peroxisome proliferator-activated receptor gamma and CCAAT-enhancer-binding protein alpha. The osteogenic markers type Ⅰ collagen, runt-related transcription factor 2, and vitamin D receptor were also upregulated on keratin substrates. Therefore, keratin can serve as a biological derived material for surface modification and scaffold fabrication for biomedical purpose. The combination of keratin with stem cells may be a potential candidate for tissue repair in the field of regenerative medicine.
机译：从人类头发中分离出来的角蛋白因其与不同细胞相互作用的内在能力而赢得了研究者的兴趣。角蛋白具有用作可控细胞外基质蛋白的潜力，可用于证明细胞机制和细胞-基质相互作用。然而，尚未报道角蛋白对干细胞的作用。在本研究中，证明了角蛋白对猪脂肪干细胞（pASCs）和一系列选择性细胞系的作用：3T3成纤维细胞，Madin-Darby犬肾（MOCK）细胞和MG63成骨细胞。相对于未处理的培养板，结果表明角蛋白包被基质促进了细胞粘附和增殖至上述细胞系。角蛋白还改善了pASC的粘附，增殖和增强的细胞活力。遗传标记的评估表明，可以成功地诱导pASCs的成脂和成骨分化，这表明角蛋白不影响pASCs的严厉性。此外，就脂蛋白脂肪酶，过氧化物酶体增殖物激活的受体γ和CCAAT增强剂结合蛋白α的上调而言，角蛋白改善了成脂分化。角蛋白基质上的成骨标记物Ⅰ型胶原蛋白，矮子相关转录因子2和维生素D受体也被上调。因此，角蛋白可以用作生物来源的材料，用于生物医学目的的表面修饰和支架制造。角蛋白与干细胞的组合可能是再生医学领域中组织修复的潜在候选者。

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