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首页> 外文会议>World biomaterials congress >Organo-selenium polymerized into a polyurethane catheter tubing inhibits biofilm formation of both gram-negative and gram-positive bacteria
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Organo-selenium polymerized into a polyurethane catheter tubing inhibits biofilm formation of both gram-negative and gram-positive bacteria

机译：聚合到聚氨酯导管中的有机硒抑制革兰氏阴性菌和革兰氏阳性菌的生物膜形成

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The mortality rate among patients with catheter-related bacterial infections (CRBSIs) reaches as high as 35%. Pathogenic bacteria colonize the surfaces of intravenous catheters, including hemodialysis catheters, and develop biofilms. Biofilms are defined as microbial-derived, sessile communities within which bacteria! cells are irreversibly attached to a substratum, an interface, or to one another. The Gram-positive opportunistic pathogen, Staphylococcus aureus, and the Gram-negative bacteria E. coli, which develops biofilms on the surfaces of different catheters, is two of the main causes of CRBSI. Therefore, it is essential to develop strategies to prevent the development of catheter-associated biofilms. We previously demonstrated that selenium (Se) in a coating on biomaterials effectively inhibits bacterial attachment and biofilm formation in vitro. This is based on the ability of selenium to be attached to organic molecules and still retain its ability to catatyticaily generate superoxide radicals. R-Se-+ 2O2 + 2R'-S- goes to RSe- + O2-. + R'S-S-R' In this study, we investigated the effectiveness of organo-selenium polymerized into the polymer of polyurethane catheter material, in preventing S. aureus and E. coli biofilm in vitro. Biofilms were developed using 1 cm pieces of the catheter and S. aureus strain AH133, and E. coli strain MM294, which express green fluorescent protein. Biofilms were visualized using confocal laser scanning microscopy (CLSM). CLSM revealed the production of a mature well-developed biofilm on the outer and inner surfaces of the control catheters. Figure 1 Lumen of a catheter tubing (no treatment) tested with S. aureus AH133. Mature biofilm present. Figure 2. Lumen of a catheter tubing (Organo-Selenium copolymer) tested with S. aureus AH133. No biofilm present Using the COMSTAT program, we quantified the biofilms by measuring substratum coverage, the mean thickness, and the mean height. This also allowed us to present three-dimensional reconstructions of the biofilms. On the outer and inner surfaces of untreated catheters, well-structured biofilms were visualized with both S. aureus AH133 and E. coli MM294, however the Figures shown above are only for S. aureus. In contrast, no bacteria attached to either surface of the Se-coated catheters. Long term stability studies of the catheters were also carried out, as well as colony forming unit assays, which showed that the catheter could be in solution for months with no loss of activity.
机译：导管相关细菌感染（CRBSI）患者的死亡率高达35％。病原菌会定居在包括血液透析导管在内的静脉导管表面，并形成生物膜。生物膜被定义为细菌衍生的微生物来源的无柄群落！细胞不可逆地附着于基质，界面或彼此。革兰氏阳性机会病原体金黄色葡萄球菌和革兰氏阴性细菌大肠杆菌在不同导管表面形成生物膜，是造成CRBSI的两个主要原因。因此，必须制定策略来防止导管相关生物膜的发展。我们先前证明了生物材料涂层中的硒（Se）在体外可有效抑制细菌附着和生物膜形成。这是基于硒附着在有机分子上的能力，并且仍然保留其催化生成超氧自由基的能力。 R-Se- + 2O2 + 2R'-S-转到RSe- + O2-。 + R'S-S-R'在这项研究中，我们研究了有机硒聚合到聚氨酯导管材料的聚合物中在预防金黄色葡萄球菌和大肠杆菌生物膜方面的有效性。使用1厘米长的导管，表达绿色荧光蛋白的金黄色葡萄球菌AH133菌株和大肠杆菌MM294菌株对生物膜进行显影。使用共聚焦激光扫描显微镜（CLSM）可视化生物膜。 CLSM揭示了在对照导管的外表面和内表面上产生了成熟的，成熟的生物膜。图1用金黄色葡萄球菌AH133测试的导管管腔（未处理）。存在成熟的生物膜。图2.用金黄色葡萄球菌AH133测试的导管管腔（Organo-Selenium共聚物）的内腔。不存在生物膜使用COMSTAT程序，我们通过测量基质覆盖率，平均厚度和平均高度来量化生物膜。这也使我们能够呈现生物膜的三维重建。在未经处理的导管的内外表面上，用金黄色葡萄球菌AH133和大肠杆菌MM294都可以观察到结构良好的生物膜，但是上面显示的数字仅适用于金黄色葡萄球菌。相反，没有细菌附着在Se涂层导管的任一表面上。还进行了导管的长期稳定性研究以及菌落形成单位测定，结果表明导管可以在溶液中放置数月，而不会丧失活性。

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