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首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Design of Scaffold with Array of Micro Projections to Trace Intra- and Inter-cellular Behavior
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Design of Scaffold with Array of Micro Projections to Trace Intra- and Inter-cellular Behavior

机译：具有微投影阵列的支架设计以追踪细胞内和细胞间行为

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The transparent micro-patterned scaffold has been designed to observe the interaction between cultured cells in vitro. The scaffold consists of a thin cover sheet and arrangement of micro-pillars. The pillars of polydimethylsiloxane were made by the photolithography technique. Each pillar has the cylindrical shape (0.007 mm diameter, 0.002 mm height). The distance between adjacent pillars is 0.003 mm. The thickness of the base sheet of the pillars is 0.02 mm. The sheet with pillars is turned upside down, and used for the scaffold of cell culture. The pillars simultaneously play a roll of the position marker. L929 (fibroblast connective tissue of mouse), or C2C12 (mouse myoblast) was seeded on the sheet at the counter surface to the pillars at the density of 1000 cells/cm~2. The cells were cultured in the medium containing 10% FBS (fetal bovine serum) and 1 % penicillin/ streptomycin. Cells are able to be traced through the transparent scaffold, but the deformation of scaffold cannot be detected. The designed scaffold has a potential to trace the intracellular and intercellular behavior microscopically in vitro.
机译：设计了透明的微图案支架，以观察体外培养细胞之间的相互作用。支架由薄的盖板和微型支柱组成。聚二甲基硅氧烷的支柱是通过光刻技术制造的。每个支柱均为圆柱形（直径0.007毫米，高度0.002毫米）。相邻立柱之间的距离为0.003毫米。支柱的基片的厚度为0.02mm。将具有支柱的薄片翻转过来，并用于细胞培养的支架。支柱同时播放一卷位置标记。将L929（小鼠的成纤维细胞结缔组织）或C2C12（小鼠成肌细胞）以1000个细胞/ cm〜2的密度播种在与支柱相对的表面上的薄片上。将细胞在含有10％FBS（胎牛血清）和1％青霉素/链霉素的培养基中培养。可以通过透明支架追踪细胞，但是无法检测到支架的变形。设计的支架具有在体外显微镜下追踪细胞内和细胞间行为的潜力。

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