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首页> 外文会议>International biohydrometallurgy symposium >Genomic Characterization of the Arsenic-Tolerant Actinobacterium, Rhodococcus erythropolis S43
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Genomic Characterization of the Arsenic-Tolerant Actinobacterium, Rhodococcus erythropolis S43

机译：耐砷放线菌红球菌S43的基因组表征

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Rhodococcus erythropolis S43 is an actinobacterium isolated from an arsenic-contaminated soil sample, collected from an old smelter site, including an arsenic smelter, in Germany. This strain has unique features as compared to the other members of the species, namely resistance to elevated concentrations of arsenic. Here, we present the microbiological features and genomic properties of this biotechnologically relevant strain. The 6,812,940 bp draft genome is arranged into 264 scaffolds of 848 contigs. It possesses 62.5% of CG content and comprises 6,040 coding sequences and 49 tRNA genes. Bioinformatic genome analysis showed the presence of arsenic-resistance genes. A complete ars operon was found containing the arsACDR cluster coding for ArsA (efflux pump ATPase), ArsC (arsenate reductase), ArsD (chaperone) and ArsR (ars operon regulator). Our results show that the arsC mRNA level significantly increased in response to arsenite and arsenate exposure, suggesting its involvement in the arsenic resistance phenotype of strain S43. In addition, this strain showed to have a plethora of genes coding for proteins involved in oxidative-stress response, including catalase, super-oxide dismutase, glutathione peroxidase-related genes, thioredoxin and thioredoxin reductase, suggesting it is highly tolerant to oxidative conditions. Finally, genes for radiation resistance, biodesulfurization, and oil and phenol degrading pathways were also detected. Altogether this data make R. erythropolis S43 a good candidate microorganism for bioremediation of highly contaminated environments and other industrial applications.
机译：Rhodococcus erythropolis S43是从砷污染的土壤样品中分离出的放线菌，该土壤样品是从德国一个古老的冶炼厂（包括一个砷冶炼厂）采集的。与该物种的其他成员相比，该菌株具有独特的特征，即对砷浓度升高的抗性。在这里，我们介绍了这种生物技术相关菌株的微生物学特征和基因组特性。 6,812,940 bp的原始基因组被安排在848个重叠群的264个支架中。它具有62.5％的CG含量，包含6,040个编码序列和49个tRNA基因。生物信息基因组分析表明存在抗砷基因。发现了完整的ars操纵子，其中包含编码ArsA（外排泵ATPase），ArsC（砷酸还原酶），ArsD（伴侣）和ArsR（ars操纵子调节子）的arsACDR簇。我们的结果表明，响应于亚砷酸盐和砷酸盐暴露，arsC mRNA水平显着增加，表明其参与了菌株S43的抗砷性表型。此外，该菌株显示出大量的基因编码与氧化应激反应有关的蛋白质，包括过氧化氢酶，超氧化物歧化酶，谷胱甘肽过氧化物酶相关的基因，硫氧还蛋白和硫氧还蛋白还原酶，表明它对氧化条件具有高度的耐受性。最后，还检测了抗辐射，生物脱硫以及油和酚降解途径的基因。总的来说，这些数据使R. erythropolis S43成为对高污染环境和其他工业应用进行生物修复的良好候选微生物。

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