Gas perfusable microfabricated membranes for high-density cell culture

机译：用于高密度细胞培养的可气体灌装的微加工膜

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We report for the first time the design, fabrication, and feasibility of a high-density cell culture system capable of a cell substrate density of 40 cm2/cm3, greater than 250× current commercially available systems. This design provides oxygen directly from gas perfusable membranes on which the cells are grown to overcome oxygen delivery limitations in traditional culture systems. With translation in mind, the membranes are composed only of medical grade PDMS and Parylene to mitigate contaminant leaching concerns.

机译：我们首次报告了高密度细胞培养系统的设计，制造和可行性，该系统的细胞底物密度为40 cm2 / cm3，大于当前市售系统的250倍。这种设计直接从气体可渗透膜中提供氧气，在该气体可渗透膜上生长细胞以克服传统培养系统中的氧气输送限制。考虑到平移，该膜仅由医用级PDMS和聚对二甲苯组成，以减轻污染物的浸出问题。

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《IEEE International Conference on Micro Electro Mechanical Systems》|2017年|472-475|共4页
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Colin A. Cook; Yang Liu; Jianming Lu; Nanhai Chen; Yuman Fong; Yu-Chong Tai;
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Biomembranes; Media; Bioreactors; Oxygen; Permeability; Fabrication; Production;

机译：生物膜;介质;生物反应器;氧气;通透性;制造;生产;

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专利

1. A Microfabricated Electrochemical Oxygen Generator for High-Density Cell Culture Arrays [J] . Michel M. Maharbiz, William J. Holtz, Sahar Sharifzadeh, Journal of Microelectromechanical Systems . 2003,第5期

机译：用于高密度细胞培养阵列的微细电化学氧气发生器
2. Gas-Permeable Membranes and Co-Culture with Fibroblasts Enable High-density Hepatocyte Culture as Multilayered Liver Tissues [J] . Fanny Evenou, Morgan Hamon, Teruo Fujii Biotechnology Progress . 2011,第4期

机译：透气膜和与成纤维细胞的共培养可作为多层肝组织进行高密度肝细胞培养
3. Expression of genes involved in drug metabolism differs between perfusable 3D liver tissue and conventional 2D‐cultured hepatocellular carcinoma cells [J] . Nobuhito Mori, Yasuyuki S. Kida FEBS Open Bio . 2020,第10期

机译：参与药物代谢的基因的表达在灌注的3D肝组织和常规的2D培养的肝细胞癌细胞之间不同
4. Gas perfusable microfabricated membranes for high-density cell culture [C] . Colin A. Cook, Yang Liu, Jianming Lu, IEEE International Conference on Micro Electro Mechanical Systems . 2017

机译：用于高密度细胞培养的气体灌注的微制酶膜
5. Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells. [D] . Qi, Hanshi Nianmin. 2002

机译：长期高密度动物细胞灌注培养中的膜和微分散通气。
6. Microfabricated Polyacrylamide Devices for the Controlled Culture of Growing Cells and Developing Organisms [O] . Philippe Nghe, Sarah Boulineau, Sebastian Gude, -1

机译：超细聚丙烯酰胺装置用于受控培养生长中的细胞和发育中的生物
7. A Microfabricated Electrochemical Oxygen Generator for High-Density Cell Culture Arrays [O] . M.M. Maharbiz, W.J. Holtz, S. Sharifzadeh, 2002

机译：用于高密度细胞培养阵列的微制造电化学氧气发生器

Gas perfusable microfabricated membranes for high-density cell culture

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