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Surface-enhanced Raman spectroscopy analysis of DNA bases using arrayed and single dimer of gold nanoparticle

机译:使用金纳米粒子的阵列和单二聚体对DNA碱基进行表面增强拉曼光谱分析

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This paper reports an ultrasensitive surface-enhanced Raman spectroscopy (SERS) for detecting and identifying four kinds of DNA bases: adenine, cytosine, thymine, and guanine using a gold nanoparticle dimer. The gold nanoparticle dimer was directionally arrayed on a substrate in order to achieve huge enhancement according to the polarization-dependent enhancement. 10-11 M limit of detection for four DNA bases and 0.1 s rapid detection for adenine were achieved. Additionally, a separated single dimer was fabricated and evaluated. We clarified single-molecule-level SERS detection and identification was possible using the locally enhanced single hotspot of the single dimer with the 1-nm nanogap.
机译:本文报道了一种超灵敏的表面增强拉曼光谱(SERS),该方法使用金纳米粒子二聚体检测和鉴定四种DNA碱基:腺嘌呤,胞嘧啶,胸腺嘧啶和鸟嘌呤。将金纳米颗粒二聚体定向排列在基底上,以便根据偏振相关的增强实现巨大的增强。四个DNA碱基的检出限为10-11 M,腺嘌呤的检出速度为0.1 s。另外,制造并评估了分离的单个二聚体。我们阐明了使用具有1 nm纳米间隙的单二聚体的局部增强的单热点,可以进行单分子水平的SERS检测和鉴定。

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