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首页> 外文会议>Society for Biomaterials annual meeting and exposition >Gradient immobilization of Platelet-derived growth factor on a nanofiber surface by controlled dopamine polymerization for tendon-bone tissue engineering
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Gradient immobilization of Platelet-derived growth factor on a nanofiber surface by controlled dopamine polymerization for tendon-bone tissue engineering

机译：肌腱-骨组织工程中可控的多巴胺聚合将血小板衍生的生长因子梯度固定在纳米纤维表面

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In this study, we immobilized PDGF growth factor on electrospun random and aligned nanofiber using polydopamine chemistry and analyzed the influence of multiple factors on ADSCs tenogenic commitment. We found that PD coating on nanofibers ensured excellent retention of immobilized PDGF and bioactivity even after 14 days of incubation. Characterization of tenogenic differentiation of ADSCs showed that aligned nanofiber with immobilized PDGF was superior in inducing tenogenic differentiation than other nanofibers. We then fabricated a PDGF gradient aligned nanofiber by utilizing the oxygen dependent dopamine polymerization for mimicking bone-tendon insertion site. Studies showed that PDGF that was immobilized in gradient manner on nanofiber able to spatially control the ADSCs phenotype similar to tendon-bone insertion site. Hence, we conclude that biochemical cues along with proper topographical cues are necessary for the efficient tenogenic differentiation of ADSCs and gradient presentation of PDGF using PD chemistry can be used as tool for stem cell based tendon-bone tissue engineering. This work was supported by a N ational Research Foundation of Korea grant funded by the Korean government (NRF-20I6R1A2B3009936).
机译：在这项研究中，我们使用聚多巴胺化学将PDGF生长因子固定在电纺随机和排列的纳米纤维上，并分析了多种因素对ADSCs肌腱定性的影响。我们发现，即使在孵育14天后，纳米纤维上的PD涂层也可以确保固定化PDGF的出色保留和生物活性。 ADSCs的肌腱分化的表征表明，与固定的PDGF对齐的纳米纤维在诱导肌腱分化方面比其他纳米纤维优越。然后，我们通过利用氧依赖性多巴胺聚合来模拟骨腱插入位点，制备了PDGF梯度排列的纳米纤维。研究表明，以梯度方式固定在纳米纤维上的PDGF能够在空间上控制类似于肌腱-骨插入位点的ADSCs表型。因此，我们得出结论，生化线索以及适当的地形线索对于ADSC的有效肌腱分化是必要的，并且使用PD化学的PDGF梯度呈递可以用作基于干细胞的腱-骨组织工程的工具。这项工作得到了由韩国政府资助的韩国国家研究基金会的资助（NRF-20I6R1A2B3009936）。

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