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首页> 外文会议>IEEE Portuguese Meeting in Bioengineering >Spectral Biomarkers of Genotoxicity from Methanol Extracts of Blood
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Spectral Biomarkers of Genotoxicity from Methanol Extracts of Blood

机译：血液甲醇提取物的遗传毒性谱生物标志物

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It is relevant to develop new monitoring techniques of carcinogenic risk associated to environmental exposition to genotoxic chemicals. The conventional biomonitoring techniques are based on laborious, expensive methods as the ones requiring isolation of lymphocytes from peripheral blood, in vitro cell culture, followed by e.g. cytokinesis-block assay and microscope observation of chromosomal abnormalities. The present work evaluated an infrared spectroscopy method, based on a simple, more economic and high-throughput procedure of analysis of whole blood processed with methanol. It was possible to identify ratios of spectral bands that are statistically different between hospital professionals occupationally exposed to antineoplastic drugs, such as 5-fluorouracil and non-hospital professionals without this exposure. It was also identified ratios of spectral bands which are statistically different between participants presenting lymphocytes with chromosomal abnormalities (as micronucleus, nuclear buds and nucleoplasmatic bridges) and participants not presenting these abnormalities. The infrared spectroscopy-based method presents therefore appealing characteristics to be applied in more intensive and/or large-scale studies of monitoring genotoxic risks.
机译：开发与环境暴露于遗传毒性化学物质有关的致癌风险的新监测技术具有重要意义。常规的生物监测技术是基于费力的，昂贵的方法，因为这些方法需要从外周血中分离出淋巴细胞，进行体外细胞培养，然后再进行例如体外培养。胞质分裂阻滞测定法和显微镜观察染色体异常。本工作基于一种简单，经济，高通量的甲醇处理全血分析程序，对红外光谱法进行了评估。可以确定在职业上接触过抗肿瘤药物的医务人员（例如5-氟尿嘧啶）和非医院医务人员之间在统计学上不同的光谱带比率。还确定了呈现带染色体异常淋巴细胞（如微核，核芽和核质桥）的参与者与未呈现这些异常的参与者之间在统计学上不同的光谱带比率。因此，基于红外光谱的方法具有吸引人的特性，可用于监测遗传毒性风险的更深入和/或更大规模的研究中。

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