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首页> 外文会议>IEEE Portuguese Meeting in Bioengineering >Novel methodology for quick detection of bacterial metabolites
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Novel methodology for quick detection of bacterial metabolites

机译：快速检测细菌代谢产物的新方法

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摘要

Fast detection of biological contaminations is of great importance in several areas of human health, such as, toxicology, environmental health and medicine. The need for developing sample retrieving methods in situ and techniques for fast and online detection of biological contamination is of crucial importance, not only for preventing infections but also to perform the most appropriate and effective medical treatment. In this work we report a method and an experimental protocol developed for the detection and analysis of VOCs emitted from bacteria by Ion Mobility Spectrometry coupled to Gas Chromatography (GC-IMS). As a biological model for study, the two Gram-Negative bacteria, Escherichia coli and Pseudomonas aeruginosa, were chosen. VOC profiles for these bacteria, individual and in the mixture, were obtained from the analysis of GC-IMS spectra. In addition, a study of the influence of the nutrition medium were performed.
机译：快速检测生物污染物在人类健康的若干领域中至关重要，例如毒理学，环境健康和医学。快速开发和在线检测生物污染的原位样品检索方法和技术的需求至关重要，这不仅是为了预防感染，而且是执行最适当和有效的医学治疗的关键。在这项工作中，我们报告了一种通过离子迁移谱-气相色谱法（GC-IMS）检测和分析细菌排放的VOC的方法和实验规程。作为研究的生物学模型，选择了两种革兰氏阴性细菌，即大肠杆菌和铜绿假单胞菌。从GC-IMS光谱分析中获得了这些细菌（单个和混合物中）的VOC曲线。另外，进行了营养培养基的影响的研究。

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