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首页> 外文会议>IEEE International Conference on Nanotechnology >Visualization of DNA Translocation and Clogging Using Photoluminescent-Free Silicon Nanopore Arrays
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Visualization of DNA Translocation and Clogging Using Photoluminescent-Free Silicon Nanopore Arrays

机译：使用无光致发光硅纳米孔阵列的DNA易位和堵塞的可视化

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摘要

Solid-state nanopore arrays hold promises for high-throughput optical analysis of single molecules. However, the high photoluminescence (PL) background emanating from the commonly used silicon nitride (SiNx) membrane for nanopore fabrication and the nonspecific adsorption of analyte on the pore sidewalls have plagued the high sensing sensitivity and efficiency offered by optical sensing. Here, the present work demonstrates an optical monitoring system using a truncated pyramidal nanopore array on a silicon membrane coated with a lipid bilayer for visualization of DNA translocation events. The silicon membrane produces essentially no PL under blue-green laser illumination, which enables more clear identification of DNA translocation and clogging events than using SiNx-based devices. The lipid bilayer coating based on small unilamellar vesicles (SUVs) minimizes the nonspecific adsorption of DNA. With confocal microscopy, the fluorescent labeled DNA translocation motion is visualized in three dimensions. The statistical results show that the percentage of DNA clogged pores is significantly reduced for the lipid bilayer coated nanopores as compared to the uncoated nanopores.
机译：固态纳米孔阵列有望实现单分子的高通量光学分析。但是，通常用于纳米孔制造的氮化硅（SiNx）膜发出的高光致发光（PL）背景以及孔侧壁上分析物的非特异性吸附已经困扰着光学传感提供的高传感灵敏度和效率。在这里，本工作演示了一种光学监控系统，该系统在涂有脂质双层的硅膜上使用截短的锥体纳米孔阵列以观察DNA易位事件。硅膜在蓝绿色激光照射下基本不产生PL，与使用基于SiNx的设备相比，它可以更清晰地识别DNA易位和堵塞事件。基于小的单层囊泡（SUVs）的脂质双层涂层可最大程度地减少DNA的非特异性吸附。使用共聚焦显微镜，可以在三个维度上观察荧光标记的DNA易位运动。统计结果表明，与未涂覆的纳米孔相比，脂质双层涂覆的纳米孔的DNA堵塞孔的百分比显着降低。

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