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首页> 外文会议>Nanoscale imaging, sensing, and actuation for biomedical applications XI >Highly-sensitive measurement of single DNA translocation through an ultraviolet light spot on silicon nanopore
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Highly-sensitive measurement of single DNA translocation through an ultraviolet light spot on silicon nanopore

机译：通过硅纳米孔上的紫外线光斑对DNA易位的高灵敏测量

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摘要

Nanopore-based sensing is an attractive candidate for developing single-molecule DNA sequencing technology. Recently, optical detection with a parallel nanopore array has been demonstrated. Although this method is a promising approach to develop high thorough-put measurement, the approach requires observation at low-background condition. In this paper, we propose a new optical method for nanopore DNA sequencing with high resolution and a high signal-to-noise ratio. We use ultraviolet light for the excitation of a fluorescent probe and a nanopore in a silicon membrane. Because silicon has a large refractive index and an extinction coefficient at ultraviolet wavelengths, light transmission thorough the membrane is negligible. This contributes to low background measurement of fluorescence from fluorophore-labeled DNA strands. In addition, the z-polarization component of the electric field is attributed to generating a large electric field gradient at the nanopore exit due to its boundary condition at the silicon surface. Our numerical electromagnetic simulation revealed that the z-component electric field was dominant compared to the x-component electric filed. The intensity of the electric field increased steeply in 2 nm, when ultraviolet light of 375nm wavelength was focused on a 10nm-thick silicon membrane with a 7 nm-diameter nanopore. This steeply increasing electric field can be sufficient resolution for the sequencing of designed DNA polymer. Finally, our experimental results demonstrated optical detection of single DNA translocation events with a high signal-to-noise ratio under applied voltage.
机译：基于纳米孔的传感技术是开发单分子DNA测序技术的诱人候选人。最近，已经证明了具有平行纳米孔阵列的光学检测。尽管此方法是开发高通量测量的有前途的方法，但该方法需要在低背景条件下进行观察。在本文中，我们提出了一种新的光学方法，用于高分辨率和高信噪比的纳米孔DNA测序。我们使用紫外线激发荧光探针和硅膜中的纳米孔。因为硅在紫外线波长下具有大的折射率和消光系数，所以穿过膜的光透射率可以忽略不计。这有助于降低荧光团标记的DNA链的荧光背景测量。另外，电场的z极化分量归因于由于其在硅表面的边界条件而在纳米孔出口处产生大的电场梯度。我们的电磁数值模拟表明，与x分量电场相比，z分量电场占主导地位。当将375nm波长的紫外光聚焦在直径为7nm的纳米孔径为10nm的硅膜上时，电场强度在2nm处急剧增加。急剧增加的电场可以为设计DNA聚合物的测序提供足够的分辨率。最后，我们的实验结果证明了在施加电压的情况下具有高信噪比的单DNA易位事件的光学检测。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    Hirohito Yamazaki; Shinji Kimura; Mutsumi Tsukahara; Shintaro Ito; Keiko Esashika; Toshiharu Saiki;

  • 作者单位

    Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa, 223-8522, Japan;

    Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa, 223-8522, Japan;

    Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa, 223-8522, Japan;

    Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa, 223-8522, Japan;

    Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa, 223-8522, Japan;

    Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa, 223-8522, Japan;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    nanopore; near-field optics; nano-photonics; ultraviolet light; DNA sequencing;

    机译：纳米孔近场光学纳米光子学紫外光线; DNA测序;

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