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Characterization of DNA translocation through a silicon based nanopore.

机译：通过硅基纳米孔的DNA易位的表征。

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摘要

The direct characterization, detection, and identification of single nucleic acid molecules can revolutionize genomics, expression analysis, and many areas of biological sciences, such as nano-medicine and nano-system biology. A nanopore fabricated using micro and nano-fabrication technique is a viable sensor for such single molecule studies. The idea is essentially a counter-counter based approach, in which the electric detection is achieved by blocking of the ionic current in the system when the species of interest passes through the single pore. Here we report the fabrication and processes characterization of silicon-based nanopore channels using e-beam lithography and (1) TEM based and (2) FESEM based processes. Subsequently, DNA measurements were performed with the nanopore as a function of potassium chloride concentrations and applied electrophoretic biases. We surprisingly observed both upwards and downwards pulses upon the translocation of DNA through the nanopore channels with varying bulk solution ionic concentration. At low ionic concentrations, the DNA resulted in increase in current whereas with higher ionic concentrations, the DNA resulted in a decrease in current. The experimental results will be presented and the explanations will be provided for the observed phenomena.
机译：单个核酸分子的直接表征，检测和鉴定可以彻底改变基因组学，表达分析以及生物科学的许多领域，例如纳米医学和纳米系统生物学。使用微米和纳米制造技术制造的纳米孔是用于此类单分子研究的可行传感器。这个想法本质上是一种基于计数器的方法，其中当目标物质通过单个孔时，通过阻止系统中的离子电流来实现电检测。在这里，我们报告使用电子束光刻和（1）基于TEM和（2）基于FESEM的工艺对硅基纳米孔通道的制造和工艺表征。随后，将纳米孔作为氯化钾浓度和施加的电泳偏差的函数进行DNA测量。我们意外地观察到DNA穿过纳米孔通道的DNA移位时，向上和向下的脉冲均具有变化的整体溶液离子浓度。在低离子浓度下，DNA导致电流增加，而在高离子浓度下，DNA导致电流减少。将给出实验结果并为观察到的现象提供解释。

著录项

  • 作者

    Chang, Hung.;

  • 作者单位

    Purdue University.;

  • 授予单位 Purdue University.;
  • 学科 Engineering Electronics and Electrical.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 144 p.
  • 总页数 144
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 无线电电子学、电信技术;
  • 关键词

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