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Enhanced Bioaccumulation of Heavy Metals by bacterial Cells with Surface-Displayed Synthetic Phytochelatins

机译：通过表面展示的合成植物丁腈通过细菌细胞增强了重金属的生物累积

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A novel strategy using synthetic phytochelatins was described for the purpose of developing microbial adsorbents for enhanced bioaccumulation of toxic metals. Synthetic genes encoding for several metal-chelating phytochelatin analogs (Glu-Cys)_nGly (ECs) were synthesized and displayed on the surface of E. coli using different anchor systems. Cells displaying ECs exhibited chain-length dependent increase in cadmium accumulation. A similar increase in Hg~(2+) accumulation was observed with cells expressing EC20 on the surface. The ability to genetically engineer ECs with precisely defined chain length could provide an attractive strategy for developing high-affinity bioadsorbents suitable for heavy metal removal.

机译：用于开发微生物吸附剂的目的，描述了一种使用合成植物切菜蛋白的新策略以提高有毒金属的生物累积。合成了用于几种金属螯合植物素类似物（Glu-Cys）_ngly（ECS）的合成基因被合成并使用不同的锚固系统在大肠杆菌表面上显示。显示ECS的细胞表现出镉积聚的链长依赖性增加。用表达表面EC20的细胞观察到Hg〜（2+）积累的类似增加。具有精确定义的链长的转基因ECS的能力可以为开发适合于重金属去除的高亲和力生物吸水剂提供有吸引力的策略。

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《ACS national meeting》|2002年||共8页
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Wilfred Chen; Weon Bae; Rajesh Mehra; Ashok Mulchandani;
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中图分类环境化学;
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1. Cell surface display of synthetic phytochelatins using ice nucleation protein for enhanced heavy metal bioaccumulation [J] . Bae W, Mulchandani A, Chen W Journal of Inorganic Biochemistry: An Interdisciplinary Journal . 2002,第2期

机译：利用冰核蛋白增强重金属生物积累的合成植物螯合素的细胞表面展示
2. Cytosolic expression of synthetic phytochelatin and bacterial metallothionein genes in Deinococcus radiodurans R1 for enhanced tolerance and bioaccumulation of cadmium [J] . Ruchi Chaturvedi, G. Archana BioMetals: An International Journal on the Role of Metal Ions in Biology, Biochemistry and Medicine . 2014,第3期

机译：合成植物螯合素和细菌金属硫蛋白基因在放射性球菌R1中的胞浆表达，以增强镉的耐受性和生物积累
3. Synthetic phytochelatins complement a phytochelatin-defkient Arabidopsis mutant and enhance the accumulation of heavy metal(loid)s [J] . Devesh Shukla, Manish Tiwari, Rudra D. Tripathi, Biochemical and Biophysical Research Communications . 2013,第3期

机译：合成植物螯合物补充了植物螯合物防御性拟南芥突变体，并增强了重金属（胶体）的积累
4. Enhanced Bioaccumulation of Heavy Metals by bacterial Cells with Surface-Displayed Synthetic Phytochelatins [C] . Wilfred Chen, Weon Bae, Rajesh Mehra, ACS national meeting . 2002

机译：通过表面展示的合成植物丁腈通过细菌细胞增强了重金属的生物累积
5. Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent. [D] . Wang, Lei. 2003

机译：固定磁铁矿的细菌细胞系统用于从电镀废水中去除和回收重金属的研究。
6. Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides [O] . Pavel Kotrba, Lucie Dolečková, Víctor de Lorenzo, 1999

机译：由于短金属结合肽的表面展示细菌细胞增强了重金属离子的生物积累
7. Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides [O] . Pavel Kotrba, Lucie Dolečková, Víctor de Lorenzo, 1999

机译：由于短金属结合肽的表面显示，细菌细胞增强了重金属离子的生物累积

Enhanced Bioaccumulation of Heavy Metals by bacterial Cells with Surface-Displayed Synthetic Phytochelatins

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