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首页> 外文会议>Conference on optical diagnostics of living cells >Rapid discrimination of silica and heavy metal oxide-coated dust by induction of changes in Ca2+i pHi and plasma membrane potential in alveolar macrophages using flow cytometry
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Rapid discrimination of silica and heavy metal oxide-coated dust by induction of changes in Ca2+i pHi and plasma membrane potential in alveolar macrophages using flow cytometry

机译：通过流式细胞术诱导肺泡巨噬细胞中Ca2 + i pHi和质膜电位的变化，快速区分二氧化硅和重金属氧化物涂层的粉尘

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Abstract: Inhalable particulate dusts are involved in the genesis of several lung diseases. Besides the well-known toxic dusts, i.e. asbestos and quartz, heavy metal-containing pollutants are considered as possible harmful substances. In the present study we compared the effect of silica chemically coated with certain metal oxides on several cell physiological parameters of bovine alveolar macrophages (BAM). The cytosolic free calcium concentration $LB@(Ca$+2$PLU$/)$-i$/$RB@, the intracellular pH (pH$-i$/), and the plasma membrane potential (MP) of BAM were measured by flow cytometry whereas the dust- induced secretion of reactive oxygen species (ROS) was measured enzymatically. Compared to control incubations with pure silica the dust-induced secretion of ROS by BAM was not affected when the particles were coated with Cr$-2$/O$-3$/, NiO, and Fe$-3$/O$-4$/, whereas VO$-2$/-coated dust induced a marked increase in ROS release. This effect was not correlated to changes in (Ca$+2$PLU$/)$-i$/, pH$-i$/, or MP. On the other hand Cr$-2$/O$-3$/-coated silica caused alterations in all of the three latter parameters. The same pattern of changes has been reported previously for quartz dusts (Tarnok et al., Anal. Cell Pathol., 15:61-72, 1997). We conclude that cell physiological measurements by flow cytometry could extend the pallet of tools to evaluate possible toxic effects of environmental dust samples. !22
机译：摘要：可吸入颗粒物粉尘与多种肺部疾病的发生有关。除了众所周知的有毒粉尘（即石棉和石英）外，还认为含重金属的污染物可能是有害物质。在本研究中，我们比较了化学镀有某些金属氧化物的二氧化硅对牛肺泡巨噬细胞（BAM）几个细胞生理参数的影响。 BAM的胞质游离钙浓度$ LB @（Ca $ + 2 $ PLU $ /）$-i $ / $ RB @通过流式细胞术测定，而通过酶法测量粉尘诱导的活性氧（ROS）分泌。与纯硅胶的对照温育相比，当颗粒用Cr $ -2 $ / O $ -3 $ /，NiO和Fe $ -3 $ / O $-包被时，BAM的粉尘诱导的ROS分泌不受影响。 4 $ /，而VO $ -2 $ /的粉尘则导致ROS释放量显着增加。此影响与（Ca $ + 2 $ PLU $ /）$-i $ /，pH $ -i $ /或MP的变化无关。另一方面，Cr $ -2 $ / O $ -3 $ /涂层的二氧化硅导致后三个参数全部发生变化。先前已经报道了石英粉尘的相同变化模式（Tarnok等人，Anal.Cell Pathol。，15：61-72，1997）。我们得出的结论是，通过流式细胞仪进行细胞生理学测量可以扩展工具的评估范围，以评估环境粉尘样品可能产生的毒性作用。 ！22

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