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首页> 外文期刊>Cytometry: The Journal of the Society for Analytical Cytology >Rapid screening of possible cytotoxic effects of particulate air pollutants by measurement of changes in cytoplasmic free calcium, cytosolic pH, and plasma membrane potential in alveolar macrophages by flow cytometry
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Rapid screening of possible cytotoxic effects of particulate air pollutants by measurement of changes in cytoplasmic free calcium, cytosolic pH, and plasma membrane potential in alveolar macrophages by flow cytometry

机译：通过流式细胞术测量肺泡巨噬细胞中胞质游离钙，胞质pH和质膜电位的变化，快速筛选颗粒状空气污染物的可能的细胞毒性作用

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Background: Inhalable particulate dusts are involved in the genesis of several lung diseases. Besides the well-known toxic dusts, i.e., asbestos and quartz, heavy metal-containing pollutants are considered as possible harmful substances. In the present study, we compared the effect of silica chemically coated with certain metal oxides and dusts from industrial productions on cell physiological parameters of bovine alveolar macrophages (BAM). Methods: The cytosolic free calcium concentration, [Ca2+](i), the intracellular pH (pH(i)), and the plasma membrane potential (MP) of BAM were measured by flow cytometry. The dust-induced secretion of reactive oxygen species (ROS) was measured enzymatically. Results: Compared with control incubations with pure silica, the dust-induced secretion of ROS by BAM was not affected when the particles were coated with Cr2O3, NiO, and Fe3O4, whereas VO2-coated dust induced a marked increase in ROS release. This effect was not correlated to changes in [Ca2+](i), pH(i), or MP. On the other hand, Cr2O3- coated silica caused alterations in all of the three latter parameters. The same pattern of changes has been reported previously for quartz dusts (Tarnok et al.: Anal Cell Pathol 15:61-72, 1997). Conclusions: We conclude that cell physiological measurements by flow cytometry could extend the palette of tools to evaluate possible toxic effects of environmental dust samples. (C) 2001 Wiley-Liss, Inc. [References: 22]
机译：背景：吸入性粉尘与几种肺部疾病的发生有关。除了众所周知的有毒粉尘（即石棉和石英）外，还认为含重金属的污染物可能是有害物质。在本研究中，我们比较了化学涂覆有某些金属氧化物和工业生产粉尘的二氧化硅对牛肺泡巨噬细胞（BAM）细胞生理参数的影响。方法：流式细胞仪检测BAM的胞浆游离钙浓度，[Ca2 +]（i），细胞内pH（pH（i））和质膜电位（MP）。用酶法测量粉尘诱导的活性氧（ROS）的分泌。结果：与纯二氧化硅的对照培养相比，当颗粒涂有Cr2O3，NiO和Fe3O4时，BAM的粉尘诱导的ROS分泌不受影响，而VO2包被的粉尘导致ROS释放显着增加。此效果与[Ca2 +]（i），pH（i）或MP的变化无关。另一方面，Cr2O3涂层的二氧化硅导致后三个参数全部发生变化。先前已经报道了石英粉尘的相同变化模式（Tarnok等人：Anal Cell Pathol 15：61-72，1997）。结论：我们得出的结论是，通过流式细胞术进行细胞生理学测量可以扩展工具范围，以评估环境粉尘样品的可能的毒性作用。 （C）2001 Wiley-Liss，Inc. [参考：22]

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