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首页> 外文会议>Saratov Fall Meeting >Quantitative spectral light scattering polarimetry for monitoring fractal growth pattern of Bacillus thuringiensis bacterial colonies
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Quantitative spectral light scattering polarimetry for monitoring fractal growth pattern of Bacillus thuringiensis bacterial colonies

机译：用于监测芽孢杆菌细菌菌落的分形生长模式的定量光谱光散射极化法

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摘要

It is of considerable current interest to develop various methods which help to understand and quantify the cellular association in growing bacterial colonies and is also important in terms of detection and identification of a bacterial species. A novel approach is used here to probe the morphological structural changes occurring during the growth of the bacterial colony of Bacillus thuringiensis under different environmental conditions (in normal nutrient agar, in presence of glucose - acting as additional nutrient and additional 3mM arsenate as additional toxic material). This approach combines the quantitative Mueller matrix polarimetry to extract intrinsic polarization properties and inverse analysis of the polarization preserving part of the light scattering spectra to determine the fractal parameter H (Hurst exponent) using Born approximation. Interesting differences are observed in the intrinsic polarization parameters and also in the Hurst exponent, which is a measurement of the fractality of a pattern formed by bacteria while growing as a colony. These findings are further confirmed with optical microscopic studies of the same sample and the results indicate a very strong and distinct dependence on the environmental conditions during growth, which can be exploited to quantify different bacterial species and their growth patterns.
机译：开发各种方法具有相当大的目的兴趣，有助于理解和量化生长细菌菌落中的细胞关联，并且在检测和鉴定细菌种类方面也很重要。这里使用一种新方法来探讨在不同的环境条件下（以正常营养琼脂在葡萄糖存在下，抑制芽孢杆菌的细菌菌落生长期间发生的形态结构变化（以葡萄糖在含有额外的营养素和另外的3mm作为额外的毒物）。该方法结合了定量穆勒矩阵偏振偏振物，提取了光散射光谱的偏振部分的内在偏振特性和逆分析，以确定使用近似的分形参数H（HURST指数）。在内在偏振参数中观察到有趣的差异，也在狭长的指数中观察到，这是一种测量由细菌形成的图案的变形，同时生长为殖民地。通过相同样品的光学微观研究进一步证实了这些发现，结果表明，在生长期间对环境条件的依赖性非常强烈，不同，这可以利用来定量不同的细菌种类及其生长模式。

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