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首页> 外文会议>Bioceramics >Can Basic Fibroblast Growth Factor Pre-Treatment Enhance Mesenchymal Stem Cell Therapy in Undecorticated Posterior Spinal Fusion?
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Can Basic Fibroblast Growth Factor Pre-Treatment Enhance Mesenchymal Stem Cell Therapy in Undecorticated Posterior Spinal Fusion?

机译：碱性成纤维细胞生长因子的预处理可以增强未变性脊柱后路融合术中的间充质干细胞治疗吗？

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Basic fibroblast growth factor (bFGF) has been shown to maintain the osteogenicity of bone marrow derived mesenchymal stem cell (MSCs) in vitro. This study was to investigate whether bFGF with osteogenic supplements could enhance bone formation of posterior spinal fusion. Rabbit bone marrow derived mesenchymal stem cells were selected by adherence on plastic culture-ware. The MSCs were exposed to dexamethasone with (bFGF group, n=6) or without bFGF (OS group, n=6). Treated cells of two groups were seeded on β -tricalcium phosphate ceramics for one day and then implanted onto L5 and L6 transverse processes of the same animal in posterior spinal fusion without decortication. The ceramics acted as control (n=6). Three fluorochromes were injected sequentially as tetracycline at week 2, xylenol orange at week 4 and calcein at week 6. The spinal segments were harvested at week 7. The bone mineral content (BMC) and volume of transverse processes was measured by peripheral quantitative computed tomography. The specimens were underwent undecalcified histology. The mineralization process was examined by fluorescent microscopy. The BMC of transverse processes in OS group was 16% greater than bFGF and control group significantly. The volume of transverse process in OS and bFGF group was significantly greater than control group by 54% and 46% respectively. The volume of transverse processes in OS group was 6% greater than bFGF group though not statistically significant. In histology, newly formed bone grew from two processes towards each other resulting in a relatively short gap distance in OS and bFGF group while less regenerated bone was observed in the control group. At the mineralization front, calcein which was injected into animal at week 6, was predominately labeled in bFGF group. In OS group, both xylenol orange (at week 4) and calcein labeled were found. In conclusion, mesenchymal stem cells pre-exposed to bFGF were not found to give additional enhancement effect on bone formation in the posterior spinal fusion model.
机译：已显示碱性成纤维细胞生长因子（bFGF）在体外可维持骨髓来源的间充质干细胞（MSCs）的成骨性。这项研究旨在调查bFGF与成骨补充剂是否可以增强后路脊柱融合术的骨形成。通过粘附在塑料培养皿上来选择兔骨髓来源的间充质干细胞。将MSCs暴露于地塞米松（bFGF组，n = 6）或不使用bFGF（OS组，n = 6）。将两组处理过的细胞接种在β-磷酸三钙陶瓷上一天，然后在不进行脱髓鞘的情况下将其植入到同一动物的L5和L6横突中。陶瓷用作对照（n ＝ 6）。在第2周，第4周，二甲苯酚橙和第6周，依次注射三种荧光染料四环素。在第7周收获脊髓节段。通过外周定量计算机断层扫描测量骨矿物质含量（BMC）和横突的体积。对标本进行未脱钙的组织学检查。通过荧光显微镜检查矿化过程。 OS组横突的BMC显着高于bFGF和对照组16％。 OS和bFGF组的横突量分别比对照组大54％和46％。 OS组的横突体积比bFGF组大6％，尽管无统计学意义。在组织学上，新形成的骨骼从两个过程向彼此长出，导致OS和bFGF组的间隙距离相对较短，而对照组中再生的骨骼较少。在矿化前沿，在第6周注射钙黄绿素的动物主要标记在bFGF组中。在OS组中，发现了二甲酚橙（第4周）和钙黄绿素标记的。总之，未发现预先暴露于bFGF的间充质干细胞在后路脊柱融合模型中对骨形成具有额外的增强作用。

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