Today the extensive application of pesticides in agriculture generates toxicological and environmental problems. Conventional analytical methods are time consuming and expensive. An alternative approach is the measurement of pesticides with optical biosensors. In this study an optical enzyme sensor for the determination of carbofuran as an example of a toxic pesticide is described. Pesticides influence an immobilized enzyme by inhibition of its activity in a test reaction (small change in pH value in a low concentrated phosphate buffer).
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