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首页> 外文会议>International Mechanical Engineering Congress and Exposition 2007 >FABRICATION OF FREE-STANDING POLYMER MEMBRANES VIA IMPRINT LITHOGRAPHY AND SELECTIVE IMMOBILIZATION OF LIPID VESICLES AT MICROPORES IN THE MEMBRANE
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FABRICATION OF FREE-STANDING POLYMER MEMBRANES VIA IMPRINT LITHOGRAPHY AND SELECTIVE IMMOBILIZATION OF LIPID VESICLES AT MICROPORES IN THE MEMBRANE

机译：通过光刻技术制备游离聚合物膜并在膜中的微孔处选择性地固定脂质囊泡

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This study presents fabrication of free-standing, perforated membranes in polymer with the pore diameter down to 500 ran via novel imprint lithography and use of the membrane to selectively immobilize lipid vesicles at the micropores in the membrane. For the fabrication, a combination of imprint lithography and a sacrificial layer technique was employed in order to get a clean, fully released, and mechanically stable membrane with perforated pores. Si molds with microscale pillar structures fabricated via photolithography were used to define patterns in SU-8 resist layer which was spin-coated on lift-off resist (LOR) used as a sacrificial layer. This was followed by a UV curing process to achieve enough mechanical strength in the SU-8 layer to be fee-standing. Release of the SU-8 layer from the LOR sacrificial layer by lift-off results in the free-standing, perforated membranes with pore diameter down to sub-micrometer range. Prior to the application of lipid vesicles, the SU-8 membrane surface was treated with poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) which prevents non-specific adsorption of the lipid vesicles on the membrane surface. As a result, lipid vesicles were found to selectively adsorb at the pore sites in the membrane, as observed with an evanescence fluorescence microscope. This result indicates that the perforated polymer membranes with micro- and nanoscale pores have potential as a platform for fundamental study of biological systems.
机译：这项研究提出了通过新颖的压印光刻技术并使用该膜选择性地将脂质囊泡固定在膜中微孔处的，孔径低至500纳米的聚合物自立式多孔膜的制造方法。对于制造，采用压印光刻和牺牲层技术的组合，以获得具有穿孔孔的清洁，完全释放和机械稳定的膜。使用通过光刻法制造的具有微型柱状结构的Si模具来定义SU-8抗蚀剂层中的图案，该图案旋涂在用作牺牲层的剥离抗蚀剂（LOR）上。随后进行紫外线固化工艺，以在SU-8层中获得足够的机械强度，以使其牢固。通过剥离将SU-8层从LOR牺牲层中释放出来，可形成孔径小于亚微米范围的自立式穿孔膜。在应用脂质囊泡之前，先用聚（L-赖氨酸）-接枝-聚（乙二醇）（PLL-g-PEG）处理SU-8膜表面，以防止脂质囊泡非特异性地吸附在脂质体上。膜表面。结果，如消逝荧光显微镜所观察到的，发现脂质囊泡选择性地吸附在膜的孔部位。该结果表明，具有微米级和纳米级孔的多孔聚合物膜具有作为生物学系统基础研究平台的潜力。

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