• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>ASME International Mechanical Engineering Congress and Exposition >FABRICATION OF FREE-STANDING POLYMER MEMBRANES VIA IMPRINT LITHOGRAPHY AND SELECTIVE IMMOBILIZATION OF LIPID VESICLES AT MICROPORES IN THE MEMBRANE
【24h】

FABRICATION OF FREE-STANDING POLYMER MEMBRANES VIA IMPRINT LITHOGRAPHY AND SELECTIVE IMMOBILIZATION OF LIPID VESICLES AT MICROPORES IN THE MEMBRANE

机译：通过压印光刻制备独立式聚合物膜和膜中微孔的脂质囊泡的选择性固定

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study presents fabrication of free-standing, perforated membranes in polymer with the pore diameter down to 500 ran via novel imprint lithography and use of the membrane to selectively immobilize lipid vesicles at the micropores in the membrane. For the fabrication, a combination of imprint lithography and a sacrificial layer technique was employed in order to get a clean, fully released, and mechanically stable membrane with perforated pores. Si molds with microscale pillar structures fabricated via photolithography were used to define patterns in SU-8 resist layer which was spin-coated on lift-off resist (LOR) used as a sacrificial layer. This was followed by a UV curing process to achieve enough mechanical strength in the SU-8 layer to be fee-standing. Release of the SU-8 layer from the LOR sacrificial layer by lift-off results in the free-standing, perforated membranes with pore diameter down to sub-micrometer range. Prior to the application of lipid vesicles, the SU-8 membrane surface was treated with poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) which prevents non-specific adsorption of the lipid vesicles on the membrane surface. As a result, lipid vesicles were found to selectively adsorb at the pore sites in the membrane, as observed with an evanescence fluorescence microscope. This result indicates that the perforated polymer membranes with micro- and nanoscale pores have potential as a platform for fundamental study of biological systems.
机译：本研究介绍了通过新颖的压印光刻和使用膜在膜中的微孔中选择性地固定在膜中微孔中的孔径的聚合物中的聚合物中的聚合物中的聚合物中的聚合物中的聚合物中的固定式膜的制造，并在膜中选择性地固定在微孔中的脂质囊泡。为了制造，采用压印光刻和牺牲层技术的组合，以便通过穿孔孔隙得到清洁，完全释放和机械稳定的膜。使用光刻法制造的微型支柱结构的Si模具用于定义SU-8抗蚀剂层中的图案，其在用作牺牲层的剥离抗蚀剂（LOR）上旋涂。接下来是UV固化过程，以在SU-8层中获得足够的机械强度以进行费用。通过剥离释放从LOR牺牲层释放SU-8层，导致独立式穿孔膜，孔径下降到亚微米范围。在施用脂质囊泡之前，用聚（L-赖氨酸） - 移植物 - 聚（乙二醇）（PLL-G-PEG）处理SU-8膜表面，这防止了脂质囊泡的非特异性吸附膜表面。结果，发现脂质囊泡在膜中的孔位点中选择性地吸附，用缺陷荧光显微镜观察到。该结果表明，具有微型和纳米尺度孔的穿孔聚合物膜具有作为生物系统对基本研究的平台。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号