• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>2011 International Conference on Remote Sensing, Environment and Transportation Engineering >Molecular cloning and bioinformatics analysis of 2, 4-dienoyl-CoA reductase1 gene in pig
【24h】

Molecular cloning and bioinformatics analysis of 2, 4-dienoyl-CoA reductase1 gene in pig

机译：猪2，4-二烯酰辅酶A还原酶1基因的分子克隆和生物信息学分析

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

2, 4-dienoyl-CoA reductase 1 (DECR1) is an auxiliary enzyme of β-oxidation, and it participates in the metabolism of polyunsaturated fatty enoyl-CoA esters. The full-length cDNA of DECR1 was first cloned from Mashen pig liver, and bioinformatics analysis were then conducted to predicted the physicochemical property, homologous analysis, modification sites and structure of DECR1 protein. Sequence analysis showed that the full-length cDNA of DECR1 was 2352 bp long with an open reading frame (ORF) of 987 bp encoding 328 amino acid residues. Homologous analysis showed that the amino acids of Mashen pig DECR1 shared 99%, 88% , 88% , 87%, 87%, 87%, 87% and 83% identity to Sus scrofa (Predicted), Bos taurus, Homo sapiens, Macaca mulatta, Pan troglodytes, Equus caballus, Canis and Mus musculus, respectively. Bioinformatics analysis showed that DECR1 was a trans-membrane protein, and molecular mass, theoretical point, aliphatic index, estimated half-life, and instability index were 35.43 kDa, 9.37, 85.70, 30 h and 29.06, respectively. Predicted DECR1 have 10 Ser, 6 Thr and 1 Tyr phosphorylation sites. Structure prediction showed that DECR1 consisted of 39.3% α-helix, 11.6% β-extended strand and 49.1% random coil in binary structure, and 2 three and a half α-helices and two 310-helices in three-dimensional structure.
机译：2、4-二壬基-CoA还原酶1（DECR1）是β-氧化的辅助酶，它参与多不饱和脂肪烯基-CoA酯的代谢。首先从马神猪肝脏中克隆出DECR1的全长cDNA，然后进行生物信息学分析以预测DECR1蛋白的理化性质，同源性分析，修饰位点和结构。序列分析显示DECR1的全长cDNA长2352 bp，带有987 bp的开放阅读框（ORF），编码328个氨基酸残基。同源分析表明，马申猪DECR1的氨基酸与Sus scrofa（预测的），Bos taurus，智人，猕猴具有99％，88％，88％，87％，87％，87％，87％和83％的同一性穆拉塔（Mulatta），盘尾猿（Pan troglodytes），马属马（Equus caballus），小犬（Canis）和小家鼠（Mus musculus）。生物信息学分析表明，DECR1是跨膜蛋白，分子量，理论点，脂肪族指数，估计半衰期和不稳定性指数分别为35.43 kDa，9.37、85.70、30 h和29.06。预测的DECR1具有10个Ser，6个Thr和1个Tyr磷酸化位点。结构预测表明，DECR1由二元结构的39.3％α-螺旋，11.6％的β-延伸链和49.1％的无规卷曲组成，在三维结构中由2个三个半α-螺旋和两个310-螺旋组成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号