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首页> 外文期刊>Indian Journal of Microbiology >Molecular Cloning and Identification of the 2'-5' Oligoadenylate Synthetase 2 Gene in Chinese Domestic Pigs Through Bioinformatics Analysis, and Determination of Its Antiviral Activity Against Porcine Reproductive and Respiratory Syndrome Virus Infection
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Molecular Cloning and Identification of the 2'-5' Oligoadenylate Synthetase 2 Gene in Chinese Domestic Pigs Through Bioinformatics Analysis, and Determination of Its Antiviral Activity Against Porcine Reproductive and Respiratory Syndrome Virus Infection

机译：通过生物信息学分析，用生物信息猪2'-5'寡替烯酸合成酶2基因的分子克隆和鉴定及其对猪生殖和呼吸综合征病毒感染的抗病毒活性的测定

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摘要

An interferon-mediated antiviral protein, 2'-5' oligoadenylate synthetase 2, plays an important role in the antiviral response of interferons. In this study, 2'-5' oligoadenylate synthetase 2 genes were cloned from Chinese domestic pigs. Bioinformatics analysis revealed that the 2024-bp long open reading fame encodes 707 amino acids. There are two conserved regions in this protein: the nucleotidyltransferase domain, and the 2'-5' oligoadenylate synthetase domain (OAS). Genetic evolution analysis showed that the 2'-5' oligoadenylate synthetase 2 gene in domestic pigs is closely related to that of cattle. There are multiple antigenic sites, no signal peptide, and no transmembrane region in the gene, which is predicted to be a hydrophilic protein. Secondary structures were found to be mainly alpha helix-based; its tertiary structure is close to that of humans and cattle, but not that of mice. Tissue distribution results indicated that this protein is distributed in multiple organs, with high distribution in the liver; it is mainly localized in the cytoplasm. PRRSV infection, interferon-beta, and Poly(I: C) treatment all promoted 2'-5' oligoadenylate synthetase 2 gene expression. Overexpression and RNA silencing of porcine OAS2 inhibited and promoted PRRSV replication in cells, respectively. The inhibitory effect of porcine OAS2 was mainly dependent on RNase L, similar to what was predicted. This study has laid the foundation for future antiviral studies in pig, and provided a new way of preventing and treating PRRSV in the future.
机译：干扰素介导的抗病毒蛋白2'-5'寡核酸合成酶2在干扰素的抗病毒反应中起重要作用。在本研究中，将2'-5'寡核酸合成酶2基因克隆来自中国国内猪。生物信息学分析显示2024-BP长开放读数的名称编码707个氨基酸。该蛋白质中有两个保守区域：核苷酰亚胺转移酶结构域和2'-5'寡核酸合成酶结构域（OAS）。遗传演进分析表明，国内猪中2'-5'寡核酸合成酶2基因与牛密切相关。存在多个抗原位点，没有信号肽，并且基因中没有跨膜区域，预测是亲水蛋白质。发现二次结构主要是基于α螺旋的;它的第三节结构与人类和牛的第三个结构接近，但不是小鼠的。组织分布结果表明该蛋白质分布在多个器官中，在肝脏中具有高分布;它主要是细胞质的局部化。 PRRSV感染，干扰素 - β和聚（I：C）治疗所有促进了2'-5'寡核酸合成酶2基因表达。猪OAS2的过表达和RNA沉默分别抑制和促进细胞中的PRRSV复制。猪OAS2的抑制作用主要取决于RNA酶L，类似于预测的内容。本研究为猪的未来抗病毒研究奠定了基础，并为未来提供了一种预防和治疗PRRSV的新方式。

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  • 来源
    《Indian Journal of Microbiology》 |2018年第3期|共13页
  • 作者

    Wang Ruining; Ma Hongfang; Kang Yinfeng; Li Cunfa; Li Huawei; Zhang Erqin; Ji Pengchao; He Jian; Zhao Mengmeng;

  • 作者单位

    Henan Univ Anim Husb &

    Econ Sch Pharmaceut Engn Zhengzhou 450046 Henan Peoples R China;

    Henan Agr Univ Coll Anim Husb &

    Vet Med Zhengzhou 450002 Henan Peoples R China;

    Sun Yat Sen Univ Collaborat Innovat Ctr Canc Med State Key Lab Oncol South China Dept Expt Res Canc Ctr Guangzhou 467500 Guangdong Peoples R China;

    Henan Univ Anim Husb &

    Econ Sch Pharmaceut Engn Zhengzhou 450046 Henan Peoples R China;

    Henan Univ Anim Husb &

    Econ Coll Biol Engn Zhengzhou 450046 Henan Peoples R China;

    Henan Agr Univ Coll Anim Husb &

    Vet Med Zhengzhou 450002 Henan Peoples R China;

    Henan Agr Univ Coll Anim Husb &

    Vet Med Zhengzhou 450002 Henan Peoples R China;

    Henan Agr Univ Coll Anim Husb &

    Vet Med Zhengzhou 450002 Henan Peoples R China;

    Henan Agr Univ Coll Anim Husb &

    Vet Med Zhengzhou 450002 Henan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    2 '-5 ' Oligoadenylate synthetase; PRRSV; RNase L; Bioinformatics; Replication;

    机译：2'5'寡核苷酸合成酶;PRRSV;RNase L;生物信息学;复制;

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