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首页> 外文会议>Academic Committee Conference of Shanghai key lab of stomatology >Decreased expression of profilin 2 in oral squamous cell carcinoma and its clinicopathological implications
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Decreased expression of profilin 2 in oral squamous cell carcinoma and its clinicopathological implications

机译：在口腔鳞状细胞癌中减少了素质2的表达及其临床病理学意义

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Profilins are small proteins essential for many normal cellular dynamics and constitute one of the crucial components of actin-based cellular motility. Several recent studies have implicated a role for the profilin (PFN) family in cancer pathogenesis and progression. However, their expression and promising functions are largely unknown in oral squamous cell carcinoma (OSCC). In this study, we analyzed the correlation between PFN1 and PFN2 expression in vitro and in vivo. The protein expression levels were roughly compared between cell lines (HIOEC, HB96) with the employment of mass spectrometry. PFN2 was singled out as one of the significantly downregulated genes in the cancerous HB96 cells. The expression levels of PFN1 and PFN2 in vitro were validated by RT-PCR, real-time PCR and Western blotting. Laser scanning confocal microscopy was used for the first time to assess the localization of PFN2 expression. In subsequent experiments, we observed the relationship between PFN2 expression levels and the proliferation of transfected HB96 cancer cells. VASP, N-WASP and P27 expression was also examined in the PFN2-transfected or non-transfected HB96 cells. In vivo, antigen expression was determined by immunohistochemical analyses in 88 paired tissue specimens. Decreased protein expression was confirmed in cancerous tissues from 88 OSCC patients compared with paracancerous normal mucous epithelia. Tumors with weak PFN2 expression were associated with a significantly worse prognosis than strongly expressed tumours (P<0.001). Other statistical analyses were performed to assess the differences in expression and their clinical and pathological significance. In conclusion, PFN2 can be utilized as both a potential suppressor marker and a prognostic protein for OSCC. The function of PFN2 may be to regulate the NWASP/Arp2/3 signaling pathway.
机译：Profilins是小分子蛋白对许多正常细胞动力学必不可少的，构成基于肌动蛋白细胞运动的重要组成部分之一。最近的几项研究已经牵连了profilin的（PFN）家族在癌症的发生和发展的作用。然而，它们的表达和有希望的功能是在口腔鳞状细胞癌（OSCC）很大程度上是未知的。在这项研究中，我们分析了在体外和体内PFN1和PFN2表达之间的相关性。蛋白质表达水平大致细胞系（HIOEC，HB96）与就业质谱之间进行比较。 PFN2挑出来作为癌症HB96细胞显著下调的基因之一。 PFN1和PFN2的体外表达水平通过RT-PCR，实时PCR和Western印迹法验证。激光扫描共聚焦显微术用于第一时间来评估PFN2表达的定位。在随后的实验中，我们观察PFN2表达水平和转染HB96癌细胞的增殖之间的关系。 VASP，N-WASP和P27的表达也进行了检查在PFN2转染或未经转染的细胞HB96。在体内，抗原表达通过免疫组织化学分析在88个配对组织样本来确定。降低蛋白质表达被证实在从88名OSCC患者癌组织与正常旁粘膜上皮比较。与PFN2表达弱肿瘤用比强烈表达肿瘤（P <0.001）预后更差显著相关联。进行其他的统计分析，以评估表达的差异及其临床和病理意义。总之，PFN2可以用作两个潜在标记抑制器和用于OSCC预后蛋白。 PFN2的功能可以是调节NWASP /的Arp2 / 3信号传导途径。

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