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首页> 外文会议>International Symposium on Information Technologies in Medicine and Education;ITME 2012 >Correlation of aberrant methylation of p16 gene to MTHFR C677T genetic polymorphisms in Hepatic Carcinoma
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Correlation of aberrant methylation of p16 gene to MTHFR C677T genetic polymorphisms in Hepatic Carcinoma

机译：肝癌p16基因异常甲基化与MTHFR C677T基因多态性的相关性。

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Object: To investigate the association between aberrant hypermethylation of p16 gene and methylenetetrahydrofolate reductase (MTHFR) genetic polymorphisms in Hepatic Carcinoma. Method: Hepatic Carcinoma cancer tissues and paracancerous normal tissues were collected. CpG island methylation status of p16 gene and MTHFR gene polymorphisms were analyzed by Real-time quantitative polymerase chain reaction (Real-time PCR). The associations between methylation status of P16 gene and clinical characteristics as well as MTHFR C677T polymorphisms were analyzed. Results: Among 86 HCC patients, the berrant hypermethylation rate of P16 gene was 60.5% in cancer tissues and 2.3% in paracancerou normal tissues. The aberrant hypermethylation rate of p16 gene in tumorous tissues was significantly higher than that in paracancerou normal tissues (χ2=67.48, p<0.05). Patients with positive HBsAg had greater significantly p16 gene methylation frequencies (χ2=5.60, p<0.05). No association was found between P16 gene methylation and selected factors including sex, age, smoking, alcohol drinking and tumor size. Compared with the MTHFR 677CC genotype, the odds ratio (OR) and 95% confidence interval (95%CI) of p16 gene with MTHFR 677T allele were 3.47(1.06 ∼ 11.37) (p<0.05). Conclution: MTHFR C677T polymorphism may be associated with hypermethylation of p16 gene, and MTHFR C677T polymorphisms may be involved in methylation-related carcinogenesis in the liver.
机译：目的：探讨肝癌中p16基因异常甲基化与亚甲基四氢叶酸还原酶（MTHFR）基因多态性的关系。方法：收集肝癌的癌组织和癌旁正常组织。通过实时定量聚合酶链反应（Real-time PCR）分析p16基因的CpG岛甲基化状态和MTHFR基因多态性。分析了P16基因甲基化状态与临床特征以及MTHFR C677T多态性之间的关联。结果：在86例HCC患者中，癌组织中P16基因的异常甲基化率分别为60.5％和癌旁正常组织中的2.3％。肿瘤组织中p16基因异常高甲基化率明显高于癌旁正常组织（χ 2 = 67.48，p <0.05）。 HBsAg阳性的患者p16基因甲基化频率明显升高（χ 2 = 5.60，p <0.05）。在P16基因甲基化与选定的因素（包括性别，年龄，吸烟，饮酒和肿瘤大小）之间未发现关联。与MTHFR 677CC基因型相比，具有MTHFR 677T等位基因的p16基因的比值比（OR）和95％置信区间（95％CI）为3.47（1.06〜11.37）（p <0.05）。结论：MTHFR C677T多态性可能与p16基因的甲基化有关，MTHFR C677T多态性可能与肝脏甲基化相关的癌变有关。

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