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Comparing Effect of Extraction Methods on Polysaccharide of Clitocybe maxima Stipe

机译:提取方法对最大砧木菌丝多糖的比较效果

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Clitocybe maxima (Gartn. ex Mey.: Fr.) Quel. is a favorable edible mushroom. As a bio-waste (or defined as byproduct), it produces a high proportion of biomass in mushroom stipe for about 45% of entire fruits. To increase its value added utilization, three extraction methods (as hot water, microwaveassisted and complex-enzyme-hydro lysis-assist) were conducted. The extraction effect on the polysaccharide of Clitocybe maxima stipe was compared and the processing conditions in extraction were optimized. The content of Clitocybe maxima polysaccharide was determined with Phenol Sulfuric Acid Method. The yield and purity of the stipe polysaccharide obtained with three extractions were greatly different, which were 5.86% and 40.23% for hot water extraction, 9.4% and 52.62% for microwareassist extraction, 10.26% and 53.13% for complexenzyme-hydrolysis extraction, respectively. Results indicated that the complex-enzyme-hydrolysis-assist extraction was the best approach for extracting the polysaccharide from Clitocybe maxima stipe with the advantages in high extraction efficiency, energy saving and running time saving.
机译:Clitocybe maxima(Gartn。ex Mey .: Fr.)奎尔。是一种可食用的蘑菇。作为一种生物废物(或定义为副产品),它在蘑菇柄中产生高比例的生物质,占整个水果的约45%。为了提高其增值利用率,进行了三种提取方法(如热水,微波辅助和复合酶水解辅助)。比较了最大菌丝对金丝桃多糖的提取效果,优化了提取工艺。采用苯酚硫酸法测定最大Clitocybe max多糖的含量。三种提取方法得到的柄柄多糖的收率和纯度差异很大,热水提取率分别为5.86%和40.23%,微助剂提取率分别为9.4%和52.62%,复合酶水解提取率分别为10.26%和53.13%。结果表明,复合酶水解辅助提取法是从最大砧木中提取多糖的最佳方法,具有提取效率高,节约能源和节省运行时间等优点。

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