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首页> 外文会议>International conference on agricultural engineering and food engineering >Selection and Evaluation of Potential Probiotic Lactic Acid Bacteria and Bifidobacteria Isolated from Human Intestinal Tract Selection and evaluation of potential probiotic
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Selection and Evaluation of Potential Probiotic Lactic Acid Bacteria and Bifidobacteria Isolated from Human Intestinal Tract Selection and evaluation of potential probiotic

机译：从人体肠道分离出的潜在益生菌乳酸菌和双歧杆菌的选择和评价潜在益生菌的选择和评价

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Three Lactobacillus strains were screened on the basis of probiotic characteristics (i.e., resistance to low pH and bile salts, adhesion to the human gastrointestinal tract, inhibition of pathogenic strains). They further exhibited producing antimicrobial activities of non-acid molecule(s). In addition, antibacterial peptides were isolated and purified from the cell-free culture supernatants of these three probiotic strains. Based on Tricine-SDSPAGE, the antimicrobial peptide was approximately 10 kDa in size. After analyzing the sequence of the 16SrDNA regions of these three strains, they were identified as Lactobacillus crispatus Lactobacillus rhamnosus and Lactobacillus rhamnosua GG. Using an in vitro system simulating gastric transit, our findings indicated that the three probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the probiotic strains inhibited subsequent adhesion of E. coli to the HT-29 cell. Among the selected strains, L. rhamnosus F1333 showed a high probiotic potential and could be used in healthpromoting food products.
机译：根据益生菌特性（即对低pH和胆汁盐的抗性，对人胃肠道的粘附性，对病原性菌株的抑制）筛选了三种乳酸杆菌菌株。它们还表现出产生非酸性分子的抗菌活性。此外，从这三种益生菌菌株的无细胞培养上清液中分离并纯化了抗菌肽。基于Tricine-SDSPAGE，抗菌肽的大小约为10 kDa。在分析这三个菌株的16SrDNA区域的序列后，它们被鉴定为crispatatus crispatus鼠李糖乳杆菌和rhamnosua GG乳杆菌。使用模拟胃运输的体外系统，我们的发现表明这三种益生菌菌株具有耐受肠胃环境的能力以及对HT-29细胞的粘附能力。证明了益生菌菌株抑制了大肠杆菌随后对HT-29细胞的粘附。在选定的菌株中，鼠李糖乳杆菌F1333具有很高的益生菌潜力，可用于健康食品。

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