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首页> 外文会议>World Congress on Medical Physics and Biomedical Engineering >In vitro Effect of Exposing Low-Frequency Electric Fields on the Proliferative Capacity and Morphological Features of Olfactory Ensheathing Cells
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In vitro Effect of Exposing Low-Frequency Electric Fields on the Proliferative Capacity and Morphological Features of Olfactory Ensheathing Cells

机译：暴露于低频电场对嗅鞘细胞增殖能力和形态学特征的体外影响

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Olfactory ensheathing cells (OECs) transplanted into the damaged spinal cord may be considered as a valuable remedy explorations for spinal cord repair. The proliferation of transplanted olfactory ensheathing cells depend on various environmental factors and effective cues, which may include electrical fields (EFs). In this study, we investigate the proliferative capacity, morphologic alterations of olfactory ensheathing cells derived from neonate rat that occur when exposed to two EFs of 20 Hz, SO mV and 20 Hz, 100 mV for 6 hours. For both EF treatments, the MTT results revealed that the cellular proliferation of exposed group during the last 6 h of the experiment were statistically higher than control. Then, we investigated morphological structure changes in the cells stained by Coomassie brilliant blue. Compare with control group, most of cells were present at intensively proliferating appearance included the microfilaments were long and thick and the accumulated appearance of cells. It is conceivable that electrical fields as a new approach may promote the growth and proliferation of OECs and may be engineered to control the survival of transplanted OECs in injured spinal cord. Although our results have been suggesting that EFs may be non-chemical strategies for cell proliferation, the fundamental mechanisms remained to be elucidated.
机译：嗅鞘细胞（OECs）移植到受损的脊髓中可以被认为是修复脊髓的有价值的治疗方法。移植的嗅鞘细胞的增殖取决于各种环境因素和有效提示，其中可能包括电场（EFs）。在这项研究中，我们调查了新生大鼠嗅鞘细胞的增殖能力，形态变化，这些变化在暴露于20 Hz，SO mV和20 Hz，100 mV的两个EF持续6小时时发生。对于两种EF治疗，MTT结果表明，在实验的最后6小时内，暴露组的细胞增殖在统计学上高于对照组。然后，我们研究了考马斯亮蓝染色的细胞的形态结构变化。与对照组相比，大多数细胞以密集增殖的形式存在，包括微丝长而浓密以及细胞的累积外观。可以想象，电场作为一种新方法可以促进OEC的生长和增殖，并且可以设计成控制移植的OEC在受损脊髓中的存活。尽管我们的结果表明，EFs可能是细胞增殖的非化学策略，但其基本机制尚待阐明。

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