首页> 外文会议>World Congress on Medical Physics and Biomedical Engineering >Fractal analysis of rat bone marrow mesenchymal stem cells during differentiation into neural cells in vitro
【24h】

Fractal analysis of rat bone marrow mesenchymal stem cells during differentiation into neural cells in vitro

机译:大鼠骨髓间充质干细胞体外分化为神经细胞过程中的分形分析

获取原文

摘要

Fractal dimension (FD) is a good option to quantitatively describe morphological complexity of cells. During the differentiation of rat bone marrow mesenchymal stem cells (rBMSC) into neural cells, morphological complexity of rBMSC increases, so FD can be a useful feature to characterize cell shape complexity at any given point in time during the differentiation process. In this study rat BMSCs were isolated and differentiated to neural cells. During the differentiation process, microscopic images were taken at certain intervals. After applying proper image processing algorithms implemented in Matlab software, FDs of the cell images were calculated by means of box counting method. Results demonstrated that FD of the recorded images had an ascending trend after one hour of neural induction, and simultaneously cells phenotype started changing from spindle shape to neural-like phenotype with multiple branches. Therefore, a direct correlation between cell shape complexity and FD of the images was observed, and it proves that FD serves as a practical method for quantitatively describing complexity as a morphological feature of the cells during differentiation.
机译:分形维数(FD)是定量描述细胞形态复杂性的好选择。在大鼠骨髓间充质干细胞(rBMSC)分化为神经细胞的过程中,rBMSC的形态复杂性增加,因此FD可能是在分化过程中任何给定时间点表征细胞形状复杂性的有用特征。在这项研究中,大鼠骨髓间充质干细胞被分离并分化为神经细胞。在分化过程中,以一定间隔拍摄显微图像。应用适用于Matlab软件的适当图像处理算法后,通过盒计数法计算细胞图像的FD。结果表明,经神经诱导一小时后,记录图像的FD呈上升趋势,同时细胞表型开始从纺锤形变为具有多个分支的神经样表型。因此,观察到细胞形状的复杂性与图像的FD之间存在直接的相关性,并且证明FD是用于定量描述作为分化过程中细胞的形态特征的复杂性的实用方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号