首页> 外文会议>Conference on digital pathology >An Automated Method for Counting Cytotoxic T-cells From CD8 Stained Images of Renal Biopsies
【24h】

An Automated Method for Counting Cytotoxic T-cells From CD8 Stained Images of Renal Biopsies

机译:从CD8染色图像计算肾活检的CD8染色图像的自动化方法

获取原文

摘要

Studying inflammatory cell subsets in transplant biopsies can be important for diagnosis and to understand pathogenesis. Counting the different subsets of lymphocytes and macrophages in the immunostained renal biopsy is often considered as the only way to characterize the inflammatory infiltrate. Counting each subset of cells in each biopsy under a light microscope can be extremely tedious, time consuming and subject to inter- and intra-personal variability. This paper presents a new method to automatically count the number of CD8 positive cytotoxic t-cells on scanned images of immunostained slides of renal allograft biopsies. The method uses normalized multi-scale difference of Gaussian to detect the potential cytotoxic t-cell candidates regions, both in the color channel and the intensity channel. Then, it fuses the information from both channels' candidate regions to detect the individual cells within cell clumps. The evaluation of the proposed method shows that there is a strong consensus between the proposed method's markings with the pathologist's markings (94.4%).
机译:研究移植活组织检查中的炎症细胞亚群对于诊断并理解发病性可能是重要的。计数免疫肾活检中的淋巴细胞和巨噬细胞的不同亚组通常被认为是表征炎症浸润的唯一方法。在光学显微镜下计算每个活组织检查中的每种细胞子集可能是极其繁琐的,耗时的耗时,并且受到间歇性的,而是受到间歇性的。本文呈现了一种新方法,用于自动计算CD8阳性细胞毒性T细胞的数量对肾同种异体移植活检的免疫载玻片的扫描图像上的扫描图像。该方法使用高斯的归一化多尺度差异检测彩色通道和强度通道中的潜在细胞毒性T细胞候选区域。然后,它使来自两个通道的候选区域的信息融合以检测单元集中的各个单元。该方法的评价表明,拟议的方法与病理学家标志的标记之间存在强烈共识(94.4%)。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号