• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International conference on applied biotechnology >Increasing Galactose Utilized Ability of Saccharomyces cerevisiae Through Gene Engineering
【24h】

Increasing Galactose Utilized Ability of Saccharomyces cerevisiae Through Gene Engineering

机译：通过基因工程提高酿酒酵母的半乳糖利用能力

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Saccharomyces cerevisiae is capable of fermenting galactose into eth-anol, but the productivity from galactose is much lower than those from glucose. An effective approach is undertaken to improve galactose utilized ability and ethanol productivity through gene engineering of the regulatory network controlling the expression of the GAL genes. The GAL gene regulatory network of S. cerevisiae is a tightly regulated system. Gal6, Gal80, and Mig1 are three known negative regulators of the GAL system. In this paper, Gal6, Gal80, and Mig1 were knockout by the way of homologous recombination. This led to a 76 % increase in specific galactose uptake rate compared with the wild-type strain. And the ethanol yield has advanced greatly. Further study showed that GAL80 and MIG1 played more important roles in galactose fermentation of S. cerevisiae than GAL6 did.
机译：Saccharomyces Cerevisiae能够将半乳糖发酵成乙醇，但是从半乳糖的生产率远低于葡萄糖的生产率。通过控制Gal基因表达的调节网络的基因工程来进行有效方法以改善半乳糖利用能力和乙醇生产率。 S.Cerevisiae的GAL基因调节网络是一个紧密调节的系统。 GAL6，GAL80和MIG1是GAL系统的三个已知负稳压器。在本文中，通过同源重组的方式敲除盖尔6，GAL80和MIG1。与野生型菌株相比，这导致特定半乳糖摄取率提高76％。乙醇产量大大提高了。进一步的研究表明，GAL80和MIG1在S.Cerevisiae的半乳糖发酵比Gal6表现出更重要的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号